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Systolic contraction within aneurysmal rabbit myocardium following transplantation of autologous skeletal myoblasts.

Publication ,  Journal Article
Emani, SM; Ellis, MJ; Dibernardo, LR; Colgrove, S; Glower, DD; Taylor, DA
Published in: J Surg Res
September 2006

OBJECTIVE: Transplantation of autologous skeletal myoblasts (SKMB) into infarcted heart (or cellular cardiomyoplasty, CCM) augments myocardial performance in animal models of myocardial infarction. However, the effect of CCM in the setting of ventricular aneurysm has not been evaluated. This study analyzes the effects of transplanted SKMB on regional wall motion in a rabbit model of postinfarct ventricular aneurysm. We hypothesize that CCM, performed early after myocardial infarction, prevents the progression of dyskinetic wall motion. METHODS: Twenty-six rabbits underwent apical left ventricular cryoinfarction and soleus muscle biopsy for in vitro isolation of skeletal myoblasts. At 2 weeks postinfarct, the presence of ventricular aneurysm was detected in 23/26 animals by sonomicrometry and micromanometry. Seventeen of 23 animals were randomized to receive either 108 autologous myoblasts (CCM) or vehicle. Regional stroke work, percent systolic shortening, and synchronicity of regional wall motion were determined prior to, and four weeks following, injection (CCM; n = 9; vehicle, n = 8). Wall motion was evaluated under baseline and stress (dobutamine, 10 (g/kg/min) conditions. Six animals did not undergo randomization, but their hearts were used to measure the size of infarction. RESULTS: Four weeks following treatment of animals with ventricular aneurysm, systolic contractile activity was present in most animals treated with myoblasts but in none treated with vehicle (5/7 versus 0/6, respectively, P < 0.05). Dobutamine tended to accentuate the differences seen at baseline between the groups. CONCLUSIONS: This study demonstrates a high incidence of systolic contractile activity in a previously aneurysmal region of myocardium following CCM and may represent a novel therapy for the prevention and treatment of postinfarct aneurysm.

Duke Scholars

Published In

J Surg Res

DOI

ISSN

0022-4804

Publication Date

September 2006

Volume

135

Issue

1

Start / End Page

202 / 208

Location

United States

Related Subject Headings

  • Transplantation, Autologous
  • Systole
  • Surgery
  • Rabbits
  • Myocardial Infarction
  • Myoblasts, Skeletal
  • Muscle, Skeletal
  • Heart Ventricles
  • Heart Aneurysm
  • Dobutamine
 

Citation

APA
Chicago
ICMJE
MLA
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Emani, S. M., Ellis, M. J., Dibernardo, L. R., Colgrove, S., Glower, D. D., & Taylor, D. A. (2006). Systolic contraction within aneurysmal rabbit myocardium following transplantation of autologous skeletal myoblasts. J Surg Res, 135(1), 202–208. https://doi.org/10.1016/j.jss.2006.03.020
Emani, Sitaram M., Matthew J. Ellis, Louis R. Dibernardo, Sharon Colgrove, Donald D. Glower, and Doris A. Taylor. “Systolic contraction within aneurysmal rabbit myocardium following transplantation of autologous skeletal myoblasts.J Surg Res 135, no. 1 (September 2006): 202–8. https://doi.org/10.1016/j.jss.2006.03.020.
Emani SM, Ellis MJ, Dibernardo LR, Colgrove S, Glower DD, Taylor DA. Systolic contraction within aneurysmal rabbit myocardium following transplantation of autologous skeletal myoblasts. J Surg Res. 2006 Sep;135(1):202–8.
Emani, Sitaram M., et al. “Systolic contraction within aneurysmal rabbit myocardium following transplantation of autologous skeletal myoblasts.J Surg Res, vol. 135, no. 1, Sept. 2006, pp. 202–08. Pubmed, doi:10.1016/j.jss.2006.03.020.
Emani SM, Ellis MJ, Dibernardo LR, Colgrove S, Glower DD, Taylor DA. Systolic contraction within aneurysmal rabbit myocardium following transplantation of autologous skeletal myoblasts. J Surg Res. 2006 Sep;135(1):202–208.
Journal cover image

Published In

J Surg Res

DOI

ISSN

0022-4804

Publication Date

September 2006

Volume

135

Issue

1

Start / End Page

202 / 208

Location

United States

Related Subject Headings

  • Transplantation, Autologous
  • Systole
  • Surgery
  • Rabbits
  • Myocardial Infarction
  • Myoblasts, Skeletal
  • Muscle, Skeletal
  • Heart Ventricles
  • Heart Aneurysm
  • Dobutamine