Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components.

Published

Journal Article

Whole-cell pertussis found in diphtheria-tetanus-pertussis (DTP) vaccine can produce symptoms reminiscent of biological responses to circulating proinflammatory monokines such as IL-6, IL-1beta, and TNFalpha. Therefore the ability of pertussis-containing vaccines and several heat-killed Bordetella pertussis preparations to stimulate cytokine production in a human monocytic cell line, THP-1, were examined. The whole-cell pertussis vaccine induced significantly more IL-6, IL-1beta, and TNFalpha production than did the acellular pertussis or diphtheria-tetanus-only vaccine. Polymyxin B was able to inhibit most of the IL-6 induced by pertussis endotoxin and a heat-killed preparation of B. pertussis containing a null mutation in bvgAS, a regulatory locus required for expression of all known protein virulence factors synthesized by this organism. However, it only partially inhibited IL-6 production induced by other pertussis-containing preparations, including DTP vaccine. These results indicate that in vitro whole-cell vaccine is a potent stimulator of IL-6, IL-1beta, and TNFalpha. They also suggest that although endotoxin is a major inducer of IL-6, other components of B. pertussis also contribute to IL-6 production by monocytes.

Full Text

Duke Authors

Cited Authors

  • Blood-Siegfried, J; Crabb Breen, E; Takeshita, S; Martinez-Maza, O; Vredevoe, D

Published Date

  • January 1998

Published In

Volume / Issue

  • 18 / 1

Start / End Page

  • 81 - 88

PubMed ID

  • 9475357

Pubmed Central ID

  • 9475357

Electronic International Standard Serial Number (EISSN)

  • 1573-2592

International Standard Serial Number (ISSN)

  • 0271-9142

Digital Object Identifier (DOI)

  • 10.1023/a:1023296022603

Language

  • eng