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Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components.

Publication ,  Journal Article
Blood-Siegfried, J; Crabb Breen, E; Takeshita, S; Martinez-Maza, O; Vredevoe, D
Published in: Journal of clinical immunology
January 1998

Whole-cell pertussis found in diphtheria-tetanus-pertussis (DTP) vaccine can produce symptoms reminiscent of biological responses to circulating proinflammatory monokines such as IL-6, IL-1beta, and TNFalpha. Therefore the ability of pertussis-containing vaccines and several heat-killed Bordetella pertussis preparations to stimulate cytokine production in a human monocytic cell line, THP-1, were examined. The whole-cell pertussis vaccine induced significantly more IL-6, IL-1beta, and TNFalpha production than did the acellular pertussis or diphtheria-tetanus-only vaccine. Polymyxin B was able to inhibit most of the IL-6 induced by pertussis endotoxin and a heat-killed preparation of B. pertussis containing a null mutation in bvgAS, a regulatory locus required for expression of all known protein virulence factors synthesized by this organism. However, it only partially inhibited IL-6 production induced by other pertussis-containing preparations, including DTP vaccine. These results indicate that in vitro whole-cell vaccine is a potent stimulator of IL-6, IL-1beta, and TNFalpha. They also suggest that although endotoxin is a major inducer of IL-6, other components of B. pertussis also contribute to IL-6 production by monocytes.

Duke Scholars

Published In

Journal of clinical immunology

DOI

EISSN

1573-2592

ISSN

0271-9142

Publication Date

January 1998

Volume

18

Issue

1

Start / End Page

81 / 88

Related Subject Headings

  • Virulence Factors, Bordetella
  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Tetanus Toxoid
  • Polymyxin B
  • Interleukin-6
  • Interleukin-1
  • Immunology
  • Humans
  • Diphtheria-Tetanus-Pertussis Vaccine
 

Citation

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Blood-Siegfried, J., Crabb Breen, E., Takeshita, S., Martinez-Maza, O., & Vredevoe, D. (1998). Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components. Journal of Clinical Immunology, 18(1), 81–88. https://doi.org/10.1023/a:1023296022603
Blood-Siegfried, J., E. Crabb Breen, S. Takeshita, O. Martinez-Maza, and D. Vredevoe. “Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components.Journal of Clinical Immunology 18, no. 1 (January 1998): 81–88. https://doi.org/10.1023/a:1023296022603.
Blood-Siegfried J, Crabb Breen E, Takeshita S, Martinez-Maza O, Vredevoe D. Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components. Journal of clinical immunology. 1998 Jan;18(1):81–8.
Blood-Siegfried, J., et al. “Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components.Journal of Clinical Immunology, vol. 18, no. 1, Jan. 1998, pp. 81–88. Epmc, doi:10.1023/a:1023296022603.
Blood-Siegfried J, Crabb Breen E, Takeshita S, Martinez-Maza O, Vredevoe D. Monokine production following in vitro stimulation of the THP-1 human monocytic cell line with pertussis vaccine components. Journal of clinical immunology. 1998 Jan;18(1):81–88.
Journal cover image

Published In

Journal of clinical immunology

DOI

EISSN

1573-2592

ISSN

0271-9142

Publication Date

January 1998

Volume

18

Issue

1

Start / End Page

81 / 88

Related Subject Headings

  • Virulence Factors, Bordetella
  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Tetanus Toxoid
  • Polymyxin B
  • Interleukin-6
  • Interleukin-1
  • Immunology
  • Humans
  • Diphtheria-Tetanus-Pertussis Vaccine