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Primary tumor genotype is an important determinant in identification of lung cancer propagating cells.

Publication ,  Journal Article
Curtis, SJ; Sinkevicius, KW; Li, D; Lau, AN; Roach, RR; Zamponi, R; Woolfenden, AE; Kirsch, DG; Wong, K-K; Kim, CF
Published in: Cell Stem Cell
July 2, 2010

Successful cancer therapy requires the elimination or incapacitation of all tumor cells capable of regenerating a tumor. Therapeutic advances therefore necessitate the characterization of the cells that are able to propagate a tumor in vivo. We show an important link between tumor genotype and isolation of tumor-propagating cells (TPCs). Three mouse models of the most common form of human lung cancer each had TPCs with a unique cell-surface phenotype. The cell-surface marker Sca1 did not enrich for TPCs in tumors initiated with oncogenic Kras, and only Sca1-negative cells propagated EGFR mutant tumors. In contrast, Sca1-positive cells were enriched for tumor-propagating activity in Kras tumors with p53 deficiency. Primary tumors that differ in genotype at just one locus can therefore have tumor-propagating cell populations with distinct markers. Our studies show that the genotype of tumor samples must be considered in studies to identify, characterize, and target tumor-propagating cells.

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Published In

Cell Stem Cell

DOI

EISSN

1875-9777

Publication Date

July 2, 2010

Volume

7

Issue

1

Start / End Page

127 / 133

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Models, Biological
  • Mice, Nude
  • Mice
  • Lung Neoplasms
  • In Vitro Techniques
  • Genotype
  • Flow Cytometry
  • Developmental Biology
  • Animals
 

Citation

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Curtis, S. J., Sinkevicius, K. W., Li, D., Lau, A. N., Roach, R. R., Zamponi, R., … Kim, C. F. (2010). Primary tumor genotype is an important determinant in identification of lung cancer propagating cells. Cell Stem Cell, 7(1), 127–133. https://doi.org/10.1016/j.stem.2010.05.021
Curtis, Stephen J., Kerstin W. Sinkevicius, Danan Li, Allison N. Lau, Rebecca R. Roach, Raffaella Zamponi, Amber E. Woolfenden, David G. Kirsch, Kwok-Kin Wong, and Carla F. Kim. “Primary tumor genotype is an important determinant in identification of lung cancer propagating cells.Cell Stem Cell 7, no. 1 (July 2, 2010): 127–33. https://doi.org/10.1016/j.stem.2010.05.021.
Curtis SJ, Sinkevicius KW, Li D, Lau AN, Roach RR, Zamponi R, et al. Primary tumor genotype is an important determinant in identification of lung cancer propagating cells. Cell Stem Cell. 2010 Jul 2;7(1):127–33.
Curtis, Stephen J., et al. “Primary tumor genotype is an important determinant in identification of lung cancer propagating cells.Cell Stem Cell, vol. 7, no. 1, July 2010, pp. 127–33. Pubmed, doi:10.1016/j.stem.2010.05.021.
Curtis SJ, Sinkevicius KW, Li D, Lau AN, Roach RR, Zamponi R, Woolfenden AE, Kirsch DG, Wong K-K, Kim CF. Primary tumor genotype is an important determinant in identification of lung cancer propagating cells. Cell Stem Cell. 2010 Jul 2;7(1):127–133.
Journal cover image

Published In

Cell Stem Cell

DOI

EISSN

1875-9777

Publication Date

July 2, 2010

Volume

7

Issue

1

Start / End Page

127 / 133

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Models, Biological
  • Mice, Nude
  • Mice
  • Lung Neoplasms
  • In Vitro Techniques
  • Genotype
  • Flow Cytometry
  • Developmental Biology
  • Animals