Imaging primary lung cancers in mice to study radiation biology.

Journal Article (Journal Article)

PURPOSE: To image a genetically engineered mouse model of non-small-cell lung cancer with micro-computed tomography (micro-CT) to measure tumor response to radiation therapy. METHODS AND MATERIALS: The Cre-loxP system was used to generate primary lung cancers in mice with mutation in K-ras alone or in combination with p53 mutation. Mice were serially imaged by micro-CT, and tumor volumes were determined. A comparison of tumor volume by micro-CT and tumor histology was performed. Tumor response to radiation therapy (15.5 Gy) was assessed with micro-CT. RESULTS: The tumor volume measured with free-breathing micro-CT scans was greater than the volume calculated by histology. Nevertheless, this imaging approach demonstrated that lung cancers with mutant p53 grew more rapidly than lung tumors with wild-type p53 and also showed that radiation therapy increased the doubling time of p53 mutant lung cancers fivefold. CONCLUSIONS: Micro-CT is an effective tool to noninvasively measure the growth of primary lung cancers in genetically engineered mice and assess tumor response to radiation therapy. This imaging approach will be useful to study the radiation biology of lung cancer.

Full Text

Duke Authors

Cited Authors

  • Kirsch, DG; Grimm, J; Guimaraes, AR; Wojtkiewicz, GR; Perez, BA; Santiago, PM; Anthony, NK; Forbes, T; Doppke, K; Weissleder, R; Jacks, T

Published Date

  • March 15, 2010

Published In

Volume / Issue

  • 76 / 4

Start / End Page

  • 973 - 977

PubMed ID

  • 20206017

Pubmed Central ID

  • PMC2847457

Electronic International Standard Serial Number (EISSN)

  • 1879-355X

Digital Object Identifier (DOI)

  • 10.1016/j.ijrobp.2009.11.038


  • eng

Conference Location

  • United States