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Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo.

Publication ,  Journal Article
Niedre, MJ; de Kleine, RH; Aikawa, E; Kirsch, DG; Weissleder, R; Ntziachristos, V
Published in: Proc Natl Acad Sci U S A
December 9, 2008

Imaging of targeted fluorescent probes offers significant advantages for investigating disease and tissue function in animal models in vivo. Conversely, macroscopic tomographic imaging is challenging because of the high scatter of light in biological tissue and the ill-posed nature of the reconstruction mathematics. In this work, we use the earliest-transmitted photons through Lewis Lung Carcinoma bearing mice, thereby dramatically reducing the effect of tissue scattering. By using a fluorescent probe sensitive to cysteine proteases, the method yielded outstanding imaging performance compared with conventional approaches. Accurate visualization of biochemical abnormalities was achieved, not only in the primary tumor, but also in the surrounding tissue related to cancer progression and inflammatory response at the organ level. These findings were confirmed histologically and with ex vivo fluorescence microscopy. The imaging fidelity demonstrated underscores a method that can use a wide range of fluorescent probes to accurately visualize cellular- and molecular-level events in whole animals in vivo.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

December 9, 2008

Volume

105

Issue

49

Start / End Page

19126 / 19131

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Tomography
  • Sensitivity and Specificity
  • Scattering, Radiation
  • Photons
  • Models, Theoretical
  • Microscopy, Fluorescence
  • Mice, Nude
  • Mice
  • Lung Neoplasms
 

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Niedre, M. J., de Kleine, R. H., Aikawa, E., Kirsch, D. G., Weissleder, R., & Ntziachristos, V. (2008). Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo. Proc Natl Acad Sci U S A, 105(49), 19126–19131. https://doi.org/10.1073/pnas.0804798105
Niedre, Mark J., Ruben H. de Kleine, Elena Aikawa, David G. Kirsch, Ralph Weissleder, and Vasilis Ntziachristos. “Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo.Proc Natl Acad Sci U S A 105, no. 49 (December 9, 2008): 19126–31. https://doi.org/10.1073/pnas.0804798105.
Niedre MJ, de Kleine RH, Aikawa E, Kirsch DG, Weissleder R, Ntziachristos V. Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo. Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19126–31.
Niedre, Mark J., et al. “Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo.Proc Natl Acad Sci U S A, vol. 105, no. 49, Dec. 2008, pp. 19126–31. Pubmed, doi:10.1073/pnas.0804798105.
Niedre MJ, de Kleine RH, Aikawa E, Kirsch DG, Weissleder R, Ntziachristos V. Early photon tomography allows fluorescence detection of lung carcinomas and disease progression in mice in vivo. Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19126–19131.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

December 9, 2008

Volume

105

Issue

49

Start / End Page

19126 / 19131

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Tomography
  • Sensitivity and Specificity
  • Scattering, Radiation
  • Photons
  • Models, Theoretical
  • Microscopy, Fluorescence
  • Mice, Nude
  • Mice
  • Lung Neoplasms