Mapping Drosophila genomic aberration breakpoints with comparative genome hybridization on microarrays.
Journal Article (Review;Journal Article)
Chromosomal aberrations are genetic "reagents" that are commonly used in Drosophila research. Stocks containing chromosomes carrying large deletions of DNA (deficiency stocks, designated Df) as well as stocks carrying an extra copy of a chromosomal region (duplication stocks, designated Dp) are essential for a variety of genetic analyses. The extent of what is deleted or duplicated has typically been determined cytologically by salivary gland polytene chromosome squashes, which identify the edges of the aberration (so-called breakpoints) of each Df or Dp at low resolution. The margin of error for this technique can be quite high, however, because it is dependent on the quality of the squash and the experience of the scientist interpreting the data. Comparative genome hybridization on microarrays provides a precise molecular method to identify which regions of the genome are deleted or duplicated in these stocks by examining a change in chromosomal ploidy across the whole genome. Furthermore, this technique allows genetic data obtained with these strains to be placed in a molecular genomic context.
Full Text
Duke Authors
Cited Authors
- Erickson, JN; Spana, EP
Published Date
- January 2006
Published In
Volume / Issue
- 410 /
Start / End Page
- 377 - 386
PubMed ID
- 16938561
Electronic International Standard Serial Number (EISSN)
- 1557-7988
International Standard Serial Number (ISSN)
- 0076-6879
Digital Object Identifier (DOI)
- 10.1016/s0076-6879(06)10018-x
Language
- eng