GMab-1, a high-affinity anti-3'-isoLM1/3',6'-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice.

Published

Journal Article

The lacto-series gangliosides 3'-isoLM1 and 3',6'-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3'-isoLM1 and 3',6'-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene beta3Gn-T5 knockout mice. In this study, we immunized beta3Gn-T5 knockout mice with 3'-isoLM1/3',6'-isoLD1 and produced the anti-3'-isoLM1/3',6'-isoLD1 mAb GMab-1, of the IgG(3) subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.

Full Text

Duke Authors

Cited Authors

  • Kato, Y; Kuan, C-T; Chang, J; Kaneko, MK; Ayriss, J; Piao, H; Chandramohan, V; Pegram, C; McLendon, RE; Fredman, P; Månsson, J-E; Bigner, DD

Published Date

  • January 1, 2010

Published In

Volume / Issue

  • 391 / 1

Start / End Page

  • 750 - 755

PubMed ID

  • 19944071

Pubmed Central ID

  • 19944071

Electronic International Standard Serial Number (EISSN)

  • 1090-2104

Digital Object Identifier (DOI)

  • 10.1016/j.bbrc.2009.11.132

Language

  • eng

Conference Location

  • United States