Genetics of drought adaptation in Arabidopsis thaliana II. QTL analysis of a new mapping population, KAS-1 x TSU-1.

Published

Journal Article

Despite compelling evidence that adaptation to local climate is common in plant populations, little is known about the evolutionary genetics of traits that contribute to climatic adaptation. A screen of natural accessions of Arabidopsis thaliana revealed Tsu-1 and Kas-1 to be opposite extremes for water-use efficiency and climate at collection sites for these accessions differs greatly. To provide a tool to understand the genetic basis of this putative adaptation, Kas-1 and Tsu-1 were reciprocally crossed to create a new mapping population. Analysis of F(3) families showed segregating variation in both delta(13)C and transpiration rate, and as expected these traits had a negative genetic correlation (r(g)=- 0.3). 346 RILs, 148 with Kas-1 cytoplasm and 198 with Tsu-1 cytoplasm, were advanced to the F(9) and genotyped using 48 microsatellites and 55 SNPs for a total of 103 markers. This mapping population was used for QTL analysis of delta(13)C using F(9) RIL means. Analysis of this reciprocal cross showed a large effect of cytoplasmic background, as well as two QTL for delta(13)C. The Kas-1 x Tsu-1 mapping population provides a powerful new resource for mapping QTL underlying natural variation and for dissecting the genetic basis of water-use efficiency differences.

Full Text

Duke Authors

Cited Authors

  • McKay, JK; Richards, JH; Nemali, KS; Sen, S; Mitchell-Olds, T; Boles, S; Stahl, EA; Wayne, T; Juenger, TE

Published Date

  • December 2008

Published In

Volume / Issue

  • 62 / 12

Start / End Page

  • 3014 - 3026

PubMed ID

  • 18691264

Pubmed Central ID

  • 18691264

Electronic International Standard Serial Number (EISSN)

  • 1558-5646

International Standard Serial Number (ISSN)

  • 0014-3820

Digital Object Identifier (DOI)

  • 10.1111/j.1558-5646.2008.00474.x

Language

  • eng