Spatiotemporal regulation of cell-cycle genes by SHORTROOT links patterning and growth.

Published

Journal Article

The development of multicellular organisms relies on the coordinated control of cell divisions leading to proper patterning and growth. The molecular mechanisms underlying pattern formation, particularly the regulation of formative cell divisions, remain poorly understood. In Arabidopsis, formative divisions generating the root ground tissue are controlled by SHORTROOT (SHR) and SCARECROW (SCR). Here we show, using cell-type-specific transcriptional effects of SHR and SCR combined with data from chromatin immunoprecipitation-based microarray experiments, that SHR regulates the spatiotemporal activation of specific genes involved in cell division. Coincident with the onset of a specific formative division, SHR and SCR directly activate a D-type cyclin; furthermore, altering the expression of this cyclin resulted in formative division defects. Our results indicate that proper pattern formation is achieved through transcriptional regulation of specific cell-cycle genes in a cell-type- and developmental-stage-specific context. Taken together, we provide evidence for a direct link between developmental regulators, specific components of the cell-cycle machinery and organ patterning.

Full Text

Duke Authors

Cited Authors

  • Sozzani, R; Cui, H; Moreno-Risueno, MA; Busch, W; Van Norman, JM; Vernoux, T; Brady, SM; Dewitte, W; Murray, JAH; Benfey, PN

Published Date

  • July 2010

Published In

Volume / Issue

  • 466 / 7302

Start / End Page

  • 128 - 132

PubMed ID

  • 20596025

Pubmed Central ID

  • 20596025

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/nature09143

Language

  • eng