Fluorescence-activated cell sorting in plant developmental biology.

Published

Journal Article

Understanding the development of an organ requires knowledge of gene, protein, and metabolite expression in the specific cell types and tissues that comprise the organ. Fluorescence-activated cell sorting (FACS) is an efficient method to isolate specific cells of interest, and the information gained from this approach has been integral to plant developmental biology. The Benfey lab has developed this method to examine gene expression profiles of different cell types in the Arabidopsis root under both standard and stress conditions. In addition to gene expression, downstream applications of FACS include proteomic and metabolite analysis. This is a powerful method to examine biological functions of specific cell types and tissues with a systems biology approach.

Full Text

Duke Authors

Cited Authors

  • Iyer-Pascuzzi, AS; Benfey, PN

Published Date

  • January 2010

Published In

Volume / Issue

  • 655 /

Start / End Page

  • 313 - 319

PubMed ID

  • 20734270

Pubmed Central ID

  • 20734270

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-60761-765-5_21

Language

  • eng