Frequent detection of bcl-2/JH translocations in human blood and organ samples by a quantitative polymerase chain reaction assay.
Journal Article (Journal Article)
Using an ultrasensitive assay involving the PCR, we have examined the frequency of a follicular lymphoma-associated translocation in peripheral blood from 132 individuals, most of whom were healthy blood donors. This translocation occurs between the bcl-2 proto-oncogene and the JH gene region and prolongs the life of lymphocytes. At a level of sensitivity of 1 translocation-bearing cell per 5 x 10(6) cells, almost one-half of healthy human adults had this translocation in the mononuclear fraction of peripheral blood. However, the range of frequency of these translocations spanned almost three orders of magnitude among translocation-positive individuals. Furthermore, there was a statistically significant increase with age in the percentage of individuals who were translocation positive. Such an age correlation was also seen for the percentage of blood donors with rather high translocation frequencies (> or = 20 per 5 x 10(6) peripheral blood mononuclear cells). However, the blood donor who had by far the highest concentration of this translocation was a healthy 35-year-old male containing approximately 900 apparently monoclonal, translocation-bearing cells per 5 x 10(6) peripheral blood mononuclear cells. Our findings suggest that some individuals who may be at risk for follicular lymphoma might be able to be identified by this PCR assay on peripheral blood. Also, these data may help explain the age dependence of the occurrence of this cancer.
Full Text
Duke Authors
Cited Authors
- Ji, W; Qu, GZ; Ye, P; Zhang, XY; Halabi, S; Ehrlich, M
Published Date
- July 1, 1995
Published In
Volume / Issue
- 55 / 13
Start / End Page
- 2876 - 2882
PubMed ID
- 7796416
International Standard Serial Number (ISSN)
- 0008-5472
Language
- eng
Conference Location
- United States