Ifremeria nautilei is deep-sea provannid gastropod endemic to hydrothermal vents at southwest Pacific back-arc spreading centers. Twelve, selectively neutral and unlinked polymorphic microsatellite loci were developed for this species. Three loci deviated significantly from Hardy-Weinberg expectations. Average observed heterozygosity ranged from 0.719 to 0.906 (mean H O = 0.547, SD = 0.206). Three of the 12 loci cross-amplified in two species of Alviniconcha (Provannidae) that co-occur with I. nautilei at Pacific vent habitats. Microsatellites developed for I. nautilei are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems. © Springer Science+Business Media B.V. 2010.