Insulin-like growth factor-1 contributes to neovascularization in age-related macular degeneration.
Journal Article (Journal Article)
Choroidal neovascularization (CNV) is a debilitating complication of age-related macular degeneration and a leading cause of vision loss. Along with other angiogenic factors like vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF)-1 and its receptor, IGF-1R, have been implicated in CNV. IGF-1 is produced in neurons and retinal pigment epithelium (RPE) but its targets and impact in CNV are not understood. IGF-1 immunoreactivity was abundant throughout surgically isolated human CNV tissues and RPE cells were immunopositive for IGF-1R. Cultured RPE cells obtained from CNV tissues expressed IGF-1R. IGF-1 stimulation of cultured cells from CNV tissues induced monophasic sustained rises in intracellular free Ca(2+). VEGF concentration in the medium of unstimulated RPE cell cultures from CNV tissues increased with time to a steady-state (8h) which was increased twofold by IGF-1 stimulation. Thus, in RPE cells IGF-1 stimulates the second messenger Ca(2+) and increases VEGF secretion which, in turn, induces neovascularization.
Full Text
Duke Authors
Cited Authors
- Rosenthal, R; Wohlleben, H; Malek, G; Schlichting, L; Thieme, H; Bowes Rickman, C; Strauss, O
Published Date
- October 29, 2004
Published In
Volume / Issue
- 323 / 4
Start / End Page
- 1203 - 1208
PubMed ID
- 15451424
Pubmed Central ID
- 15451424
International Standard Serial Number (ISSN)
- 0006-291X
Digital Object Identifier (DOI)
- 10.1016/j.bbrc.2004.08.219
Language
- eng
Conference Location
- United States