Insulin-like growth factor-1 contributes to neovascularization in age-related macular degeneration.

Published

Journal Article

Choroidal neovascularization (CNV) is a debilitating complication of age-related macular degeneration and a leading cause of vision loss. Along with other angiogenic factors like vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF)-1 and its receptor, IGF-1R, have been implicated in CNV. IGF-1 is produced in neurons and retinal pigment epithelium (RPE) but its targets and impact in CNV are not understood. IGF-1 immunoreactivity was abundant throughout surgically isolated human CNV tissues and RPE cells were immunopositive for IGF-1R. Cultured RPE cells obtained from CNV tissues expressed IGF-1R. IGF-1 stimulation of cultured cells from CNV tissues induced monophasic sustained rises in intracellular free Ca(2+). VEGF concentration in the medium of unstimulated RPE cell cultures from CNV tissues increased with time to a steady-state (8h) which was increased twofold by IGF-1 stimulation. Thus, in RPE cells IGF-1 stimulates the second messenger Ca(2+) and increases VEGF secretion which, in turn, induces neovascularization.

Full Text

Duke Authors

Cited Authors

  • Rosenthal, R; Wohlleben, H; Malek, G; Schlichting, L; Thieme, H; Bowes Rickman, C; Strauss, O

Published Date

  • October 29, 2004

Published In

Volume / Issue

  • 323 / 4

Start / End Page

  • 1203 - 1208

PubMed ID

  • 15451424

Pubmed Central ID

  • 15451424

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/j.bbrc.2004.08.219

Language

  • eng

Conference Location

  • United States