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Performance of diagnostic tests to detect respiratory viruses in older adults.

Publication ,  Journal Article
She, RC; Polage, CR; Caram, LB; Taggart, EW; Hymas, WC; Woods, CW; Schmader, K; Petti, CA
Published in: Diagn Microbiol Infect Dis
July 2010

The performance of 4 laboratory methods for diagnosis of viral respiratory tract infections (RTI) in older adults was evaluated. Seventy-four nasopharyngeal (NP) swab specimens were obtained from 60 patients with RTI at a long-term care facility over 2 respiratory seasons. Sixteen specimens were positive for a respiratory virus by at least 1 method. Multiplex reverse transcriptase polymerase chain reaction (RT-PCR) by the Luminex xTAG Respiratory Viral Panel (RVP) detected 16 (100%) of the positive specimens, RVP of 24-h culture supernatant detected 8 (50%), direct fluorescent antibody testing detected 4 (25%), rapid culture detected 2 (12.5%), and rapid antigen testing detected none. For a comparison group, RVP was performed on NP swabs from 20 outpatient children with RTI. The mean fluorescence intensity by RVP was significantly lower for positive adult patients than pediatric patients (P = 0.0373). Our data suggest that older adult patients shed lower titers of viruses, necessitating a highly sensitive assay such as RT-PCR to reliably detect respiratory viral pathogens.

Duke Scholars

Published In

Diagn Microbiol Infect Dis

DOI

EISSN

1879-0070

Publication Date

July 2010

Volume

67

Issue

3

Start / End Page

246 / 250

Location

United States

Related Subject Headings

  • Viruses
  • Virus Shedding
  • Virus Diseases
  • Virus Cultivation
  • Virology
  • Respiratory Tract Infections
  • Nasopharynx
  • Molecular Diagnostic Techniques
  • Middle Aged
  • Microbiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
She, R. C., Polage, C. R., Caram, L. B., Taggart, E. W., Hymas, W. C., Woods, C. W., … Petti, C. A. (2010). Performance of diagnostic tests to detect respiratory viruses in older adults. Diagn Microbiol Infect Dis, 67(3), 246–250. https://doi.org/10.1016/j.diagmicrobio.2010.02.020
She, Rosemary C., Christopher R. Polage, Lauren B. Caram, Edward W. Taggart, Weston C. Hymas, Christopher W. Woods, Kenneth Schmader, and Cathy A. Petti. “Performance of diagnostic tests to detect respiratory viruses in older adults.Diagn Microbiol Infect Dis 67, no. 3 (July 2010): 246–50. https://doi.org/10.1016/j.diagmicrobio.2010.02.020.
She RC, Polage CR, Caram LB, Taggart EW, Hymas WC, Woods CW, et al. Performance of diagnostic tests to detect respiratory viruses in older adults. Diagn Microbiol Infect Dis. 2010 Jul;67(3):246–50.
She, Rosemary C., et al. “Performance of diagnostic tests to detect respiratory viruses in older adults.Diagn Microbiol Infect Dis, vol. 67, no. 3, July 2010, pp. 246–50. Pubmed, doi:10.1016/j.diagmicrobio.2010.02.020.
She RC, Polage CR, Caram LB, Taggart EW, Hymas WC, Woods CW, Schmader K, Petti CA. Performance of diagnostic tests to detect respiratory viruses in older adults. Diagn Microbiol Infect Dis. 2010 Jul;67(3):246–250.
Journal cover image

Published In

Diagn Microbiol Infect Dis

DOI

EISSN

1879-0070

Publication Date

July 2010

Volume

67

Issue

3

Start / End Page

246 / 250

Location

United States

Related Subject Headings

  • Viruses
  • Virus Shedding
  • Virus Diseases
  • Virus Cultivation
  • Virology
  • Respiratory Tract Infections
  • Nasopharynx
  • Molecular Diagnostic Techniques
  • Middle Aged
  • Microbiology