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Cytoprotective effects of proteasome beta5 subunit overexpression in lens epithelial cells.

Publication ,  Journal Article
Liu, Y; Liu, X; Zhang, T; Luna, C; Liton, PB; Gonzalez, P
Published in: Mol Vis
January 16, 2007

PURPOSE: To determine whether the overexpression of the proteasome catalytic beta5 subunit (PSMB5) can induce the expression of the catalytic subunits beta1 and beta2, enhance proteasome activity, and exert a cytoprotective effect in lens epithelial cells. METHODS: Cells from the human lens epithelial cell line SRA01/04 (LECs) were stably transfected either with a plasmid expressing the proteasome catalytic subunit beta5 or with an empty plasmid. beta-5-expressing LECs and controls were analyzed for the expression of beta1, beta2, beta5, and alpha6 proteasome subunits; chymotrypsin-like (CT-L) and peptidylglutamyl-peptide hydrolase (PGPH) catalytic activities; as well as for the accumulation of carbonylated proteins, rates of cell viability, and apoptosis after oxidative stress. RESULTS: Stable expression of the beta5 proteasome subunit resulted in increased expression of the catalytic subunits beta1 and beta2, increased CT-L and PGPH proteasome activities, and increased resistance to accumulation of carbonylated proteins and cell death after oxidative stress. CONCLUSIONS: The proteasome activity can be genetically "upregulated" in lens cells by overexpression of the beta5 catalytic subunit. The resulting increase in proteasome activity leads to a decrease in the accumulation of oxidized proteins and enhanced cell survival following oxidative stress.

Duke Scholars

Published In

Mol Vis

EISSN

1090-0535

Publication Date

January 16, 2007

Volume

13

Start / End Page

31 / 38

Location

United States

Related Subject Headings

  • Protein Carbonylation
  • Proteasome Endopeptidase Complex
  • Oxidative Stress
  • Oxidation-Reduction
  • Ophthalmology & Optometry
  • Lens, Crystalline
  • Humans
  • Eye Proteins
  • Epithelial Cells
  • Endopeptidases
 

Citation

APA
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MLA
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Liu, Y., Liu, X., Zhang, T., Luna, C., Liton, P. B., & Gonzalez, P. (2007). Cytoprotective effects of proteasome beta5 subunit overexpression in lens epithelial cells. Mol Vis, 13, 31–38.
Liu, Yizhi, Xialin Liu, Tieying Zhang, Coralia Luna, Paloma B. Liton, and Pedro Gonzalez. “Cytoprotective effects of proteasome beta5 subunit overexpression in lens epithelial cells.Mol Vis 13 (January 16, 2007): 31–38.
Liu Y, Liu X, Zhang T, Luna C, Liton PB, Gonzalez P. Cytoprotective effects of proteasome beta5 subunit overexpression in lens epithelial cells. Mol Vis. 2007 Jan 16;13:31–8.
Liu, Yizhi, et al. “Cytoprotective effects of proteasome beta5 subunit overexpression in lens epithelial cells.Mol Vis, vol. 13, Jan. 2007, pp. 31–38.
Liu Y, Liu X, Zhang T, Luna C, Liton PB, Gonzalez P. Cytoprotective effects of proteasome beta5 subunit overexpression in lens epithelial cells. Mol Vis. 2007 Jan 16;13:31–38.

Published In

Mol Vis

EISSN

1090-0535

Publication Date

January 16, 2007

Volume

13

Start / End Page

31 / 38

Location

United States

Related Subject Headings

  • Protein Carbonylation
  • Proteasome Endopeptidase Complex
  • Oxidative Stress
  • Oxidation-Reduction
  • Ophthalmology & Optometry
  • Lens, Crystalline
  • Humans
  • Eye Proteins
  • Epithelial Cells
  • Endopeptidases