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The role of Toll-like receptor 4 in environmental airway injury in mice.

Publication ,  Journal Article
Hollingsworth, JW; Cook, DN; Brass, DM; Walker, JK; Morgan, DL; Foster, WM; Schwartz, DA
Published in: American journal of respiratory and critical care medicine
July 2004

Inhalation of toxins commonly found in air pollution contributes to the development and progression of asthma and environmental airway injury. In this study, we investigated the requirement of toll-like receptor 4 (TLR4) in mice for pulmonary responses to three environmental toxins: aerosolized lipopolysaccharide, particulate matter (residual oil fly ash), and ozone. The physiologic and biologic responses to these toxins were evaluated by the extent of airway responsiveness, neutrophil recruitment to the lower respiratory tract, changes in inflammatory cytokines, and the concentration of protein in the lavage fluid. Genetically engineered, TLR4-deficient mice (C57BL/6(TLR4-/-)) were unresponsive to inhaled lipopolysaccharide, except for minimal increases in some inflammatory cytokines. In contrast, C57BL/6(TLR4-/-) mice did not differ from wild-type mice in their airway response to instilled residual oil fly ash or acute ozone exposure; however, we found that, despite a robust inflammatory response, C57BL/6(TLR4-/-) mice are protected against the development of airway hyperresponsiveness after subchronic ozone exposure. These data demonstrate in the mouse that the requirement of TLR4 for pulmonary inflammation depends on the nature of the toxin and appears specific to toxin and exposure conditions.

Duke Scholars

Published In

American journal of respiratory and critical care medicine

ISSN

1073-449X

Publication Date

July 2004

Volume

170

Issue

2

Start / End Page

126 / 132

Location

united states

Related Subject Headings

  • Toll-Like Receptors
  • Toll-Like Receptor 4
  • Respiratory Tract Diseases
  • Respiratory System
  • Respiratory Physiological Phenomena
  • Receptors, Cell Surface
  • Proteins
  • Particulate Matter
  • Ozone
  • Neutrophils
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hollingsworth, J. W., Cook, D. N., Brass, D. M., Walker, J. K., Morgan, D. L., Foster, W. M., & Schwartz, D. A. (2004). The role of Toll-like receptor 4 in environmental airway injury in mice. American Journal of Respiratory and Critical Care Medicine, 170(2), 126–132.
Hollingsworth, J. W., D. N. Cook, D. M. Brass, J. K. Walker, D. L. Morgan, W. M. Foster, and D. A. Schwartz. “The role of Toll-like receptor 4 in environmental airway injury in mice.American Journal of Respiratory and Critical Care Medicine 170, no. 2 (July 2004): 126–32.
Hollingsworth JW, Cook DN, Brass DM, Walker JK, Morgan DL, Foster WM, et al. The role of Toll-like receptor 4 in environmental airway injury in mice. American journal of respiratory and critical care medicine. 2004 Jul;170(2):126–32.
Hollingsworth, J. W., et al. “The role of Toll-like receptor 4 in environmental airway injury in mice.American Journal of Respiratory and Critical Care Medicine, vol. 170, no. 2, July 2004, pp. 126–32.
Hollingsworth JW, Cook DN, Brass DM, Walker JK, Morgan DL, Foster WM, Schwartz DA. The role of Toll-like receptor 4 in environmental airway injury in mice. American journal of respiratory and critical care medicine. 2004 Jul;170(2):126–132.

Published In

American journal of respiratory and critical care medicine

ISSN

1073-449X

Publication Date

July 2004

Volume

170

Issue

2

Start / End Page

126 / 132

Location

united states

Related Subject Headings

  • Toll-Like Receptors
  • Toll-Like Receptor 4
  • Respiratory Tract Diseases
  • Respiratory System
  • Respiratory Physiological Phenomena
  • Receptors, Cell Surface
  • Proteins
  • Particulate Matter
  • Ozone
  • Neutrophils