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Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice.

Publication ,  Journal Article
Rice, HE; Hsu, EW; Sheng, H; Evenson, DA; Freemerman, AJ; Safford, KM; Provenzale, JM; Warner, DS; Johnson, GA
Published in: AJR Am J Roentgenol
April 2007

OBJECTIVE: Adipose-derived stem cells are an alternative stem cell source for CNS therapies. The goals of the current study were to label adipose-derived stem cells with superparamagnetic iron oxide (SPIO) particles, to use MRI to guide the transplantation of adipose-derived stem cells in middle cerebral artery occlusion (MCAO)-injured mice, and to localize donor adipose-derived stem cells in the injured brain using MRI. We hypothesized that we would successfully label adipose-derived stem cells and image them with MRI. MATERIALS AND METHODS: Adipose-derived stem cells harvested from mice inbred for green fluorescent protein were labeled with SPIO ferumoxide particles through the use of poly-L-lysine. Adipose-derived stem cell viability, iron staining, and proliferation were measured after SPIO labeling, and the sensitivity of MRI in the detection of SPIO-labeled adipose-derived stem cells was assessed ex vivo. Adult mice (n = 12) were subjected to unilateral MCAO. Two weeks later, in vivo 7-T MRI was performed to guide stereotactic transplantation of SPIO-labeled adipose-derived stem cells into brain tissue adjacent to the infarct. After 24 hours, the mice were sacrificed for high-resolution ex vivo 7-T or 9.4-T MRI and histologic study. RESULTS: Adipose-derived stem cells were efficiently labeled with SPIO particles without loss of cell viability or proliferation. Using MRI, we guided precise transplantation of adipose-derived stem cells. MR images of mice given injections of SPIO-labeled adipose-derived stem cells had hypointense regions that correlated with the histologic findings in donor cells. CONCLUSION: MRI proved useful in transplantation of adipose-derived stem cells in vivo. This imaging technique may be useful for studies of CNS stem cell therapies.

Duke Scholars

Published In

AJR Am J Roentgenol

DOI

EISSN

1546-3141

Publication Date

April 2007

Volume

188

Issue

4

Start / End Page

1101 / 1108

Location

United States

Related Subject Headings

  • Stem Cell Transplantation
  • Oxides
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Magnetite Nanoparticles
  • Magnetic Resonance Imaging
  • Iron
  • Infarction, Middle Cerebral Artery
  • Ferrosoferric Oxide
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rice, H. E., Hsu, E. W., Sheng, H., Evenson, D. A., Freemerman, A. J., Safford, K. M., … Johnson, G. A. (2007). Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice. AJR Am J Roentgenol, 188(4), 1101–1108. https://doi.org/10.2214/AJR.06.0663
Rice, Henry E., Edward W. Hsu, Huaxin Sheng, Debra A. Evenson, Alex J. Freemerman, Kristine M. Safford, James M. Provenzale, David S. Warner, and G Allan Johnson. “Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice.AJR Am J Roentgenol 188, no. 4 (April 2007): 1101–8. https://doi.org/10.2214/AJR.06.0663.
Rice HE, Hsu EW, Sheng H, Evenson DA, Freemerman AJ, Safford KM, et al. Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice. AJR Am J Roentgenol. 2007 Apr;188(4):1101–8.
Rice, Henry E., et al. “Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice.AJR Am J Roentgenol, vol. 188, no. 4, Apr. 2007, pp. 1101–08. Pubmed, doi:10.2214/AJR.06.0663.
Rice HE, Hsu EW, Sheng H, Evenson DA, Freemerman AJ, Safford KM, Provenzale JM, Warner DS, Johnson GA. Superparamagnetic iron oxide labeling and transplantation of adipose-derived stem cells in middle cerebral artery occlusion-injured mice. AJR Am J Roentgenol. 2007 Apr;188(4):1101–1108.

Published In

AJR Am J Roentgenol

DOI

EISSN

1546-3141

Publication Date

April 2007

Volume

188

Issue

4

Start / End Page

1101 / 1108

Location

United States

Related Subject Headings

  • Stem Cell Transplantation
  • Oxides
  • Nuclear Medicine & Medical Imaging
  • Mice, Inbred C57BL
  • Mice
  • Magnetite Nanoparticles
  • Magnetic Resonance Imaging
  • Iron
  • Infarction, Middle Cerebral Artery
  • Ferrosoferric Oxide