A comprehensive method for optical-emission computed tomography.

Journal Article

Optical-computed tomography (CT) and optical-emission computed tomography (ECT) are recent techniques with potential for high-resolution multi-faceted 3D imaging of the structure and function in unsectioned tissue samples up to 1-4 cc. Quantitative imaging of 3D fluorophore distribution (e.g. GFP) using optical-ECT is challenging due to attenuation present within the sample. Uncorrected reconstructed images appear hotter near the edges than at the center. A similar effect is seen in SPECT/PET imaging, although an important difference is attenuation occurs for both emission and excitation photons. This work presents a way to implement not only the emission attenuation correction utilized in SPECT, but also excitation attenuation correction and source strength modeling which are unique to optical-ECT. The performance of the correction methods was investigated by the use of a cylindrical gelatin phantom whose central region was filled with a known distribution of attenuation and fluorophores. Uncorrected and corrected reconstructions were compared to a sectioned slice of the phantom imaged using a fluorescent dissecting microscope. Significant attenuation artifacts were observed in uncorrected images and appeared up to 80% less intense in the central regions due to attenuation and an assumed uniform light source. The corrected reconstruction showed agreement throughout the verification image with only slight variations ( approximately 5%). Final experiments demonstrate the correction in tissue as applied to a tumor with constitutive RFP.

Full Text

Duke Authors

Cited Authors

  • Thomas, A; Bowsher, J; Roper, J; Oliver, T; Dewhirst, M; Oldham, M

Published Date

  • July 21, 2010

Published In

Volume / Issue

  • 55 / 14

Start / End Page

  • 3947 - 3957

PubMed ID

  • 20577042

Electronic International Standard Serial Number (EISSN)

  • 1361-6560

Digital Object Identifier (DOI)

  • 10.1088/0031-9155/55/14/001

Language

  • eng

Conference Location

  • England