Detection of proximal colorectal cancers through analysis of faecal DNA.

Journal Article (Letter)

Detection of mutations in faecal DNA represents a promising, non-invasive approach for detecting colorectal cancers in average-risk populations. One of the first practical applications of this technology involves the examination of microsatellite markers in sporadic cancers with mismatch-repair deficiencies. Since such cancers nearly always occur in the proximal colon, this test might be useful as an adjunct to sigmoidoscopy, which detects only distal colorectal lesions. We report here the first in-depth analysis of faecal DNA from patients with proximal cancers to determine the feasibility, sensitivity, and specificity of this approach. Using a sensitive method for microsatellite mutation detection, we found that 18 of 46 cancers had microsatellite alterations and that identical mutations could be identified in the faecal DNA of 17 of these 18 cases.

Full Text

Duke Authors

Cited Authors

  • Traverso, G; Shuber, A; Olsson, L; Levin, B; Johnson, C; Hamilton, SR; Boynton, K; Kinzler, KW; Vogelstein, B

Published Date

  • February 2, 2002

Published In

Volume / Issue

  • 359 / 9304

Start / End Page

  • 403 - 404

PubMed ID

  • 11844514

International Standard Serial Number (ISSN)

  • 0140-6736

Digital Object Identifier (DOI)

  • 10.1016/S0140-6736(02)07591-8

Language

  • eng

Conference Location

  • England