Unexpected role of clathrin adaptor AP-1 in MHC-dependent positive selection of T cells.

Journal Article

Trafficking of transmembrane receptors to a specific intracellular compartment is conducted by adaptor molecules that bind to target motifs within the cytoplasmic domains of cargo proteins. We generated mice containing a lymphoid-specific deficiency of AP-1 using RNAi knockdown technology. Inhibition of AP-1 expression in thymocytes blocks progression from double-positive immature thymocytes, resulting in complete absence of CD4(+) single-positive thymocytes and severe reduction of CD3(+)CD8(+) single-positive thymocytes. Analysis of the contribution of AP-1 deficiency on the interaction between mature CD4(+) T cells and antigen-presenting cells revealed that AP-1 is essential to efficient immune synapse formation and associated T cell activation, suggesting a possible mechanism of AP-1 function in thymocyte development.

Full Text

Duke Authors

Cited Authors

  • Alvarez Arias, DA; McCarty, N; Lu, L; Maldonado, RA; Shinohara, ML; Cantor, H

Published Date

  • February 9, 2010

Published In

Volume / Issue

  • 107 / 6

Start / End Page

  • 2556 - 2561

PubMed ID

  • 20133794

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.0913671107

Language

  • eng

Conference Location

  • United States