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Technology evaluation: Stem-cell therapy, Aastrom Biosciences Inc.

Publication ,  Journal Article
Morse, MA
Published in: Curr Opin Mol Ther
December 1999

The expansion of human stem cells and their genetic manipulation represent areas of increasing interest in the field of stem cell transplantation. Previously, stem cell transplantation has been accomplished by using cellular products obtained by large volume bone marrow or peripheral blood harvest. Difficulties with this approach include inadequate cell numbers and tumor cell contamination. Furthermore, for gene transfer modalities requiring proliferating progenitor cells, low gene expression would be expected in these products. To address these difficulties, the AastromReplicell System has been developed as a fully closed and automated system for expanding hematopoietic cells. Investigators at Aastrom have evaluated the conditions needed for optimal growth including the need for unpurified bone marrow or cord blood mononuclear cells, high cell densities, serum-containing medium and certain types of plastic surfaces. Studies have now been initiated to demonstrate the feasibility of generating enough cells to fully reconstitute hematopoiesis from small volumes of cellular progenitors. It has also been demonstrated that tumor cell contamination passively decreases during the culture period. It now remains to be shown in a direct comparison that this approach yields greater efficacy and a lower cost than transplantation with unmanipulated large volume marrow or peripheral blood stem cell products.

Duke Scholars

Published In

Curr Opin Mol Ther

EISSN

2040-3445

Publication Date

December 1999

Volume

1

Issue

6

Start / End Page

745 / 752

Location

England

Related Subject Headings

  • Transplantation, Autologous
  • Humans
  • Hematopoietic Stem Cells
  • Hematopoietic Stem Cell Transplantation
  • Hematopoiesis
  • Feasibility Studies
  • Cord Blood Stem Cell Transplantation
  • Cell Proliferation
  • Cell Culture Techniques
  • Biotechnology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Morse, M. A. (1999). Technology evaluation: Stem-cell therapy, Aastrom Biosciences Inc. Curr Opin Mol Ther, 1(6), 745–752.
Morse, M. A. “Technology evaluation: Stem-cell therapy, Aastrom Biosciences Inc.Curr Opin Mol Ther 1, no. 6 (December 1999): 745–52.
Morse MA. Technology evaluation: Stem-cell therapy, Aastrom Biosciences Inc. Curr Opin Mol Ther. 1999 Dec;1(6):745–52.
Morse, M. A. “Technology evaluation: Stem-cell therapy, Aastrom Biosciences Inc.Curr Opin Mol Ther, vol. 1, no. 6, Dec. 1999, pp. 745–52.
Morse MA. Technology evaluation: Stem-cell therapy, Aastrom Biosciences Inc. Curr Opin Mol Ther. 1999 Dec;1(6):745–752.

Published In

Curr Opin Mol Ther

EISSN

2040-3445

Publication Date

December 1999

Volume

1

Issue

6

Start / End Page

745 / 752

Location

England

Related Subject Headings

  • Transplantation, Autologous
  • Humans
  • Hematopoietic Stem Cells
  • Hematopoietic Stem Cell Transplantation
  • Hematopoiesis
  • Feasibility Studies
  • Cord Blood Stem Cell Transplantation
  • Cell Proliferation
  • Cell Culture Techniques
  • Biotechnology