Total plasmatic homocysteine and von Willebrand factor in experimental diabetes mellitus.

Journal Article (Journal Article)

OBJECTIVES: To determine the plasma homocysteine and von Willebrand factor levels as markers of endothelial dysfunction in rats with diabetes mellitus induced by streptozotocin. METHODS: Thirty-five adult male rats (Rattus norvegicus albinus) (weight between 180-200 g) were randomized into three groups: control group (n=10), which received no drugs or vehicles; sham group (n=10), which received streptozotocin solution; and diabetic group (n=15), which received streptozotocin. Eight weeks after diabetes mellitus induction, the animals were weighed and anesthesized; blood samples were collected from abdominal aorta for plasma total homocysteine, von Willebrand factor and glucose levels. RESULTS: The experimental model was reproducible in 100% of animals. The mean plasma homocysteine levels were: 7.9 micromol/l (control), 8.6 micromol/l (sham) and 6.1 micromol/l (diabetic), with difference among the groups (p<0.01). Multiple comparison analysis among the groups showed that values in the diabetic group were lower than in the sham group (p<0.01). The mean von Willebrand factor values were 0.15 U/l (control), 0.16 U/l (sham) and 0.18 U/l (diabetic), with difference among the groups (p=0.03). The mean value was higher in the diabetic group than in the control group (p<0.05). Correlation between homocysteine and von Willebrand factor was not observed in the diabetic group. CONCLUSION: Reduced homocysteine levels and increased von Willebrand factor levels were observed in diabetes mellitus induced by streptozotocin; nevertheless, there were no correlations between them and with final glucose levels.

Full Text

Duke Authors

Cited Authors

  • Lopes, RD; Neves, LB; D'Almeida, V; Conceição, GMDS; Gabriel, A

Published Date

  • April 2007

Published In

Volume / Issue

  • 88 / 4

Start / End Page

  • 424 - 429

PubMed ID

  • 17546272

Electronic International Standard Serial Number (EISSN)

  • 1678-4170

Digital Object Identifier (DOI)

  • 10.1590/s0066-782x2007000400010


  • eng por

Conference Location

  • Brazil