Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells.

Published

Journal Article

Osteopontin (Opn) contributes to diverse biological processes that include immune responses, vascularization, and bone formation. Until recently, studies describing the activities of Opn have focused on the cytokine-like properties of the secreted protein. Here, we show that alternative translation of a single Opn mRNA species generates a secreted and intracellular isoform. Utilization of a 5' canonical translation start site generates a protein that includes an N-terminal signal sequence allowing targeting to secretory vesicles and cytokine secretion, whereas usage of a downstream start site generates a shortened protein that lacks the N-terminal signal sequence and localizes mainly to cytoplasm. The coordinated action of these Opn gene products regulates the functional phenotype of subsets of dendritic cells.

Full Text

Duke Authors

Cited Authors

  • Shinohara, ML; Kim, H-J; Kim, J-H; Garcia, VA; Cantor, H

Published Date

  • May 20, 2008

Published In

Volume / Issue

  • 105 / 20

Start / End Page

  • 7235 - 7239

PubMed ID

  • 18480255

Pubmed Central ID

  • 18480255

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.0802301105

Language

  • eng

Conference Location

  • United States