Detailed analysis of gene expression during development of T cell lineages in the thymus.

Published

Journal Article

The genetic mechanisms that promote lineage commitment and eliminate autoreactive cells in the thymus are not well understood. To better understand this process, we have identified and quantitated transcripts in the two major thymocyte lineages by using serial analysis of gene expression. Approximately 25 genes displayed almost complete segregation to one or the other T cell lineage. Commitment to the CD4 lineage was marked by up-regulation of genes associated with increased survival and chaperone function followed by expression of genes that regulate nucleosome remodeling and T cell receptor signaling. Differentiation within the CD8 lineage, on the other hand, was marked by up-regulation of genes that regulate lymphocyte homing, followed by quenching of genes that inhibit apoptosis. Definition of differential gene expression during development of the two major thymocyte lineages will allow insight into mechanisms of T cell development after positive and negative selection.

Full Text

Duke Authors

Cited Authors

  • McCarty, N; Shinohara, ML; Lu, L; Cantor, H

Published Date

  • June 22, 2004

Published In

Volume / Issue

  • 101 / 25

Start / End Page

  • 9339 - 9344

PubMed ID

  • 15190182

Pubmed Central ID

  • 15190182

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0402654101

Language

  • eng

Conference Location

  • United States