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Osteopontin expression during early cerebral ischemia-reperfusion in rats: enhanced expression in the right cortex is suppressed by acetaminophen.

Publication ,  Journal Article
Baliga, SS; Merrill, GF; Shinohara, ML; Denhardt, DT
Published in: PLoS One
January 21, 2011

Osteopontin (OPN) is a pleiotropic protein implicated in various inflammatory responses including ischemia-reperfusion (I-R) injury. Two distinct forms of the protein have been identified: an extensively studied secreted form (sOPN) and a less-well-known intracellular form (iOPN). Studies have shown that increased OPN expression parallels the time course of macrophage infiltration into injured tissue, a late event in the development of cerebral infarcts. sOPN has been suggested to promote remodeling of the extracellular matrix in the brain; the function of iOPN may be to facilitate certain signal transduction processes. Here, we studied OPN expression in adult male Sprague-Dawley rats subjected to global forebrain I-R injury. We found iOPN in the cytoplasm of both cortices and the hippocampus, but unexpectedly only the right cortex exhibited a marked increase in the iOPN level after 45 min of reperfusion. Acetaminophen, a drug recently shown to decrease apoptotic incidence, caspase-9 activation, and mitochondrial dysfunction during global I-R, significantly inhibited the increase in iOPN protein in the right cortex, suggesting a role for iOPN in the response to I-R injury in the right cortex.

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

January 21, 2011

Volume

6

Issue

1

Start / End Page

e14568

Location

United States

Related Subject Headings

  • Reperfusion Injury
  • Rats
  • Osteopontin
  • General Science & Technology
  • Gene Expression
  • Cerebral Cortex
  • Brain Ischemia
  • Animals
  • Acetaminophen
 

Citation

APA
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ICMJE
MLA
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Baliga, S. S., Merrill, G. F., Shinohara, M. L., & Denhardt, D. T. (2011). Osteopontin expression during early cerebral ischemia-reperfusion in rats: enhanced expression in the right cortex is suppressed by acetaminophen. PLoS One, 6(1), e14568. https://doi.org/10.1371/journal.pone.0014568
Baliga, Sunanda S., Gary F. Merrill, Mari L. Shinohara, and David T. Denhardt. “Osteopontin expression during early cerebral ischemia-reperfusion in rats: enhanced expression in the right cortex is suppressed by acetaminophen.PLoS One 6, no. 1 (January 21, 2011): e14568. https://doi.org/10.1371/journal.pone.0014568.
Baliga, Sunanda S., et al. “Osteopontin expression during early cerebral ischemia-reperfusion in rats: enhanced expression in the right cortex is suppressed by acetaminophen.PLoS One, vol. 6, no. 1, Jan. 2011, p. e14568. Pubmed, doi:10.1371/journal.pone.0014568.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

January 21, 2011

Volume

6

Issue

1

Start / End Page

e14568

Location

United States

Related Subject Headings

  • Reperfusion Injury
  • Rats
  • Osteopontin
  • General Science & Technology
  • Gene Expression
  • Cerebral Cortex
  • Brain Ischemia
  • Animals
  • Acetaminophen