The extracellular matrix protein mindin serves as an integrin ligand and is critical for inflammatory cell recruitment.

Published

Journal Article

Leukocyte recruitment to inflammation sites depends on interactions between integrins and extracellular matrix (ECM). In this report we show that mice lacking the ECM protein mindin exhibit severely impaired recruitment of neutrophils and macrophages in 4 different inflammation models. Furthermore, neutrophils directly bind to immobilized mindin, and mindin matrix mediates neutrophil migration in vitro. The adhesion of neutrophils to mindin is blocked by anti-integrin alpha4, anti-integrin alpha(M), and anti-integrin beta2 antibodies. We also show that HEK-293 cells transfected with cDNA encoding these integrins exhibit enhanced binding to immobilized mindin matrix and the increased binding can be blocked by anti-integrin antibodies. Our results suggest that mindin serves as a novel ligand for integrins and mindin-integrin interactions are critical for inflammatory cell recruitment in vivo.

Full Text

Duke Authors

Cited Authors

  • Jia, W; Li, H; He, Y-W

Published Date

  • December 1, 2005

Published In

Volume / Issue

  • 106 / 12

Start / End Page

  • 3854 - 3859

PubMed ID

  • 16105980

Pubmed Central ID

  • 16105980

International Standard Serial Number (ISSN)

  • 0006-4971

Digital Object Identifier (DOI)

  • 10.1182/blood-2005-04-1658

Language

  • eng

Conference Location

  • United States