Consistent production of transgenic chickens using replication-deficient retroviral vectors and high-throughput screening procedures.

Published

Journal Article

We have developed a novel method of DNA extraction combined with a high-throughput method of gene detection allowing thousands of potentially transgenic chicks to be screened quickly and reliably. By using this method and a replication-deficient retroviral vector based on avian leukosis virus (ALV), we have demonstrated germline transmission of three different transgenes. Several generations of chickens carrying intact transgenes were produced, validating the use of the ALV retroviral vectors for large-scale production of transgenic flocks. Fourth-generation chicks that were nontransgenic, hemizygous, or homozygous for the transgene were identified with the combined genetic screening methods.

Full Text

Duke Authors

Cited Authors

  • Harvey, AJ; Speksnijder, G; Baugh, LR; Morris, JA; Ivarie, R

Published Date

  • February 2002

Published In

Volume / Issue

  • 81 / 2

Start / End Page

  • 202 - 212

PubMed ID

  • 11873828

Pubmed Central ID

  • 11873828

Electronic International Standard Serial Number (EISSN)

  • 1525-3171

International Standard Serial Number (ISSN)

  • 0032-5791

Digital Object Identifier (DOI)

  • 10.1093/ps/81.2.202

Language

  • eng