Glycosylation of proteinase 3 (PR3) is not required for its reactivity with antineutrophil cytoplasmic antibodies (ANCA) in Wegener's granulomatosis.

Journal Article (Multicenter Study;Journal Article)

Objective

The glycosylation status of autoantigens appears to be crucial for the pathogenesis of some autoimmune diseases, since carbohydrates play a crucial role in the distinction of self from non-self. Proteinase 3 (PR3), the main target antigen for anti-neutrophil cytoplasmic antibodies (ANCA) in patients with Wegener's granulomatosis (WG), contains two Asn-linked glycosylation sites. The present study explores the influence of the glycosylation status of PR3 on the PR3 recognition by ANCA in a well characterized population of patients with WG.

Methods

Forty-four patients with WG (459 serum samples) who participated in a multicenter randomized trial, were tested by capture ELISA for ANCA against PR3 and deglycosylated recombinant variants of PR3.

Results

The patients were followed for a median of 27 months, and the median number of serum samples per patient was 10. At baseline, the correlation between the levels of ANCA against PR3 and against all the deglycosylated recombinant variants of PR3 were greater than 0.94 (?<0.001 for all the comparisons). Longitudinal analyses comparing the levels of ANCA against PR3 versus all the deglycosylated recombinant variants of PR3, using linear mixed models, showed no significant statistical differences (rho >or=0.90 in all cases).

Conclusion

The glycosylation status of PR3 has no impact on its recognition by ANCA in WG.

Full Text

Duke Authors

Cited Authors

  • Finkielman, JD; Merkel, PA; Schroeder, D; Hoffman, GS; Spiera, R; St Clair, EW; Davis, JC; McCune, WJ; Lears, A; Ytterberg, SR; Hummel, AM; Viss, MA; Peikert, T; Stone, JH; Specks, U; WGET Research Group,

Published Date

  • January 2009

Published In

Volume / Issue

  • 27 / 1 Suppl 52

Start / End Page

  • S45 - S52

PubMed ID

  • 19646346

Pubmed Central ID

  • PMC3183098

Electronic International Standard Serial Number (EISSN)

  • 1593-098X

International Standard Serial Number (ISSN)

  • 0392-856X

Language

  • eng