Promoters, enhancers, and transcription target RAG1 binding during V(D)J recombination.

Published

Journal Article

V(D)J recombination assembles antigen receptor genes in a well-defined order during lymphocyte development. This sequential process has long been understood in the context of the accessibility model, which states that V(D)J recombination is regulated by controlling the ability of the recombination machinery to gain access to its chromosomal substrates. Indeed, many features of "open" chromatin correlate with V(D)J recombination, and promoters and enhancers have been strongly implicated in creating a recombinase-accessible configuration in neighboring chromatin. An important prediction of the accessibility model is that cis-elements and transcription control binding of the recombination-activating gene 1 (RAG1) and RAG2 proteins to their DNA targets. However, this prediction has not been tested directly. In this study, we use mutant Tcra and Tcrb alleles to demonstrate that enhancers control RAG1 binding globally at Jα or Dβ/Jβ gene segments, that promoters and transcription direct RAG1 binding locally, and that RAG1 binding can be targeted in the absence of RAG2. These findings reveal important features of the genetic mechanisms that regulate RAG binding and provide a direct confirmation of the accessibility model.

Full Text

Duke Authors

Cited Authors

  • Ji, Y; Little, AJ; Banerjee, JK; Hao, B; Oltz, EM; Krangel, MS; Schatz, DG

Published Date

  • December 20, 2010

Published In

Volume / Issue

  • 207 / 13

Start / End Page

  • 2809 - 2816

PubMed ID

  • 21115692

Pubmed Central ID

  • 21115692

Electronic International Standard Serial Number (EISSN)

  • 1540-9538

Digital Object Identifier (DOI)

  • 10.1084/jem.20101136

Language

  • eng

Conference Location

  • United States