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Promoters, enhancers, and transcription target RAG1 binding during V(D)J recombination.

Publication ,  Journal Article
Ji, Y; Little, AJ; Banerjee, JK; Hao, B; Oltz, EM; Krangel, MS; Schatz, DG
Published in: J Exp Med
December 20, 2010

V(D)J recombination assembles antigen receptor genes in a well-defined order during lymphocyte development. This sequential process has long been understood in the context of the accessibility model, which states that V(D)J recombination is regulated by controlling the ability of the recombination machinery to gain access to its chromosomal substrates. Indeed, many features of "open" chromatin correlate with V(D)J recombination, and promoters and enhancers have been strongly implicated in creating a recombinase-accessible configuration in neighboring chromatin. An important prediction of the accessibility model is that cis-elements and transcription control binding of the recombination-activating gene 1 (RAG1) and RAG2 proteins to their DNA targets. However, this prediction has not been tested directly. In this study, we use mutant Tcra and Tcrb alleles to demonstrate that enhancers control RAG1 binding globally at Jα or Dβ/Jβ gene segments, that promoters and transcription direct RAG1 binding locally, and that RAG1 binding can be targeted in the absence of RAG2. These findings reveal important features of the genetic mechanisms that regulate RAG binding and provide a direct confirmation of the accessibility model.

Duke Scholars

Published In

J Exp Med

DOI

EISSN

1540-9538

Publication Date

December 20, 2010

Volume

207

Issue

13

Start / End Page

2809 / 2816

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • Transcription, Genetic
  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, alpha-beta
  • Protein Binding
  • Promoter Regions, Genetic
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ji, Y., Little, A. J., Banerjee, J. K., Hao, B., Oltz, E. M., Krangel, M. S., & Schatz, D. G. (2010). Promoters, enhancers, and transcription target RAG1 binding during V(D)J recombination. J Exp Med, 207(13), 2809–2816. https://doi.org/10.1084/jem.20101136
Ji, Yanhong, Alicia J. Little, Joydeep K. Banerjee, Bingtao Hao, Eugene M. Oltz, Michael S. Krangel, and David G. Schatz. “Promoters, enhancers, and transcription target RAG1 binding during V(D)J recombination.J Exp Med 207, no. 13 (December 20, 2010): 2809–16. https://doi.org/10.1084/jem.20101136.
Ji Y, Little AJ, Banerjee JK, Hao B, Oltz EM, Krangel MS, et al. Promoters, enhancers, and transcription target RAG1 binding during V(D)J recombination. J Exp Med. 2010 Dec 20;207(13):2809–16.
Ji, Yanhong, et al. “Promoters, enhancers, and transcription target RAG1 binding during V(D)J recombination.J Exp Med, vol. 207, no. 13, Dec. 2010, pp. 2809–16. Pubmed, doi:10.1084/jem.20101136.
Ji Y, Little AJ, Banerjee JK, Hao B, Oltz EM, Krangel MS, Schatz DG. Promoters, enhancers, and transcription target RAG1 binding during V(D)J recombination. J Exp Med. 2010 Dec 20;207(13):2809–2816.

Published In

J Exp Med

DOI

EISSN

1540-9538

Publication Date

December 20, 2010

Volume

207

Issue

13

Start / End Page

2809 / 2816

Location

United States

Related Subject Headings

  • VDJ Recombinases
  • Transcription, Genetic
  • Recombination, Genetic
  • Receptors, Antigen, T-Cell, alpha-beta
  • Protein Binding
  • Promoter Regions, Genetic
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male