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Transcription-dependent mobilization of nucleosomes at accessible TCR gene segments in vivo.

Publication ,  Journal Article
Kondilis-Mangum, HD; Cobb, RM; Osipovich, O; Srivatsan, S; Oltz, EM; Krangel, MS
Published in: J Immunol
June 15, 2010

Accessibility of chromosomal recombination signal sequences to the RAG protein complex is known to be essential for V(D)J recombination at Ag receptor loci in vivo. Previous studies have addressed the roles of cis-acting regulatory elements and germline transcription in the covalent modification of nucleosomes at Ag receptor loci. However, a detailed picture of nucleosome organization at accessible and inaccessible recombination signal sequences has been lacking. In this study, we have analyzed the nucleosome organization of accessible and inaccessible Tcrb and Tcra alleles in primary murine thymocytes in vivo. We identified highly positioned arrays of nucleosomes at Dbeta, Jbeta, and Jalpha segments and obtained evidence indicating that positioning is established at least in part by the regional DNA sequence. However, we found no consistent positioning of nucleosomes with respect to recombination signal sequences, which could be nucleosomal or internucleosomal even in their inaccessible configurations. Enhancer- and promoter-dependent accessibility was characterized by diminished abundance of certain nucleosomes and repositioning of others. Moreover, some changes in nucleosome positioning and abundance at Jalpha61 were shown to be a direct consequence of germline transcription. We suggest that enhancer- and promoter-dependent transcription generates optimal recombinase substrates in which some nucleosomes are missing and others are covalently modified.

Duke Scholars

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

June 15, 2010

Volume

184

Issue

12

Start / End Page

6970 / 6977

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Regulatory Sequences, Nucleic Acid
  • Receptors, Antigen, T-Cell
  • Promoter Regions, Genetic
  • Nucleosomes
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kondilis-Mangum, H. D., Cobb, R. M., Osipovich, O., Srivatsan, S., Oltz, E. M., & Krangel, M. S. (2010). Transcription-dependent mobilization of nucleosomes at accessible TCR gene segments in vivo. J Immunol, 184(12), 6970–6977. https://doi.org/10.4049/jimmunol.0903923
Kondilis-Mangum, Hrisavgi D., Robin Milley Cobb, Oleg Osipovich, Sruti Srivatsan, Eugene M. Oltz, and Michael S. Krangel. “Transcription-dependent mobilization of nucleosomes at accessible TCR gene segments in vivo.J Immunol 184, no. 12 (June 15, 2010): 6970–77. https://doi.org/10.4049/jimmunol.0903923.
Kondilis-Mangum HD, Cobb RM, Osipovich O, Srivatsan S, Oltz EM, Krangel MS. Transcription-dependent mobilization of nucleosomes at accessible TCR gene segments in vivo. J Immunol. 2010 Jun 15;184(12):6970–7.
Kondilis-Mangum, Hrisavgi D., et al. “Transcription-dependent mobilization of nucleosomes at accessible TCR gene segments in vivo.J Immunol, vol. 184, no. 12, June 2010, pp. 6970–77. Pubmed, doi:10.4049/jimmunol.0903923.
Kondilis-Mangum HD, Cobb RM, Osipovich O, Srivatsan S, Oltz EM, Krangel MS. Transcription-dependent mobilization of nucleosomes at accessible TCR gene segments in vivo. J Immunol. 2010 Jun 15;184(12):6970–6977.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

June 15, 2010

Volume

184

Issue

12

Start / End Page

6970 / 6977

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Regulatory Sequences, Nucleic Acid
  • Receptors, Antigen, T-Cell
  • Promoter Regions, Genetic
  • Nucleosomes
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Immunology