IL-10-producing regulatory B10 cells inhibit intestinal injury in a mouse model.

Journal Article (Journal Article)

B cells mediate multiple functions that influence immune and inflammatory responses. In mice, the addition of dextran sulfate sodium (DSS) to drinking water leads to immediate intestinal injury. Dextran sulfate sodium-induced intestinal injury serves as an experimental animal model for human ulcerative colitis. The contribution of B cells to DSS-induced intestinal injury is unclear. In this study, we show that DSS-induced intestinal injury was more severe in CD19-deficient (CD19(-/-)) mice than in wild-type mice. These inflammatory responses were negatively regulated by a unique IL-10-producing CD1d(hi)CD5(+) regulatory B cell subset (B10 cells) that was absent in CD19(-/-) mice and represented only 1% to 2% of splenic B220(+) cells in wild-type mice. Remarkably, adoptive transfer of these B10 cells from wild-type mice reduced inflammation in CD19(-/-) mice in an IL-10-dependent manner. These results demonstrate that IL-10 production from regulatory B10 cells regulates DSS-induced intestinal injury. These findings may provide new insights and therapeutic approaches for treating ulcerative colitis.

Full Text

Duke Authors

Cited Authors

  • Yanaba, K; Yoshizaki, A; Asano, Y; Kadono, T; Tedder, TF; Sato, S

Published Date

  • February 2011

Published In

Volume / Issue

  • 178 / 2

Start / End Page

  • 735 - 743

PubMed ID

  • 21281806

Pubmed Central ID

  • PMC3069829

Electronic International Standard Serial Number (EISSN)

  • 1525-2191

Digital Object Identifier (DOI)

  • 10.1016/j.ajpath.2010.10.022


  • eng

Conference Location

  • United States