Establishment of experimental eosinophilic vasculitis by IgE-mediated cutaneous reverse passive arthus reaction.
Journal Article (Journal Article)
Prominent eosinophil infiltration is a characteristic of some forms of vasculitis, such as Churg-Strauss syndrome, also known as allergic granulomatous vasculitis. In the current study, we established a mouse model of cutaneous eosinophilic vasculitis by the cutaneous reverse passive Arthus reaction using IgE injection instead of IgG. Wild-type C57BL/6 mice were injected with IgE anti-trinitrophenyl antibodies, followed immediately by intravenous administration of trinitrophenyl bovine serum albumin. IgE-mediated immune complex challenge induced substantial hemorrhage with marked infiltration of eosinophils in which neutrophils, mast cells, and macrophages were also mixed. This finding contrasted remarkably with the neutrophil-dominant infiltration pattern in IgG-mediated immune complex challenge. In the lesion, the expression level of monocyte chemotactic protein-3 was increased, and anti-monocyte chemotactic protein-3 treatment resulted in a significant but incomplete blockade of eosinophil recruitment. Furthermore, mice lacking E-selectin, P-selectin, L-selectin, or intercellular adhesion molecule-1, as well as wild-type mice that received anti-vascular cell adhesion molecule-1-blocking antibodies were assessed for the IgE-mediated Arthus reaction. After 24 hours, the loss of P-selectin resulted in a significant reduction in eosinophil accumulation compared with both wild-type mice and other mouse mutants. Collectively, the Fc class of immunoglobulins, which forms these immune complexes, critically determines the disease manifestation of vasculitis. The IgE-mediated cutaneous reverse passive Arthus reaction may serve as an experimental model for cutaneous eosinophilic infiltration in vasculitis as well as in other diseases.
Full Text
Duke Authors
Cited Authors
- Ishii, T; Fujita, T; Matsushita, T; Yanaba, K; Hasegawa, M; Nakashima, H; Ogawa, F; Shimizu, K; Takehara, K; Tedder, TF; Sato, S; Fujimoto, M
Published Date
- June 2009
Published In
Volume / Issue
- 174 / 6
Start / End Page
- 2225 - 2233
PubMed ID
- 19389931
Pubmed Central ID
- 19389931
Electronic International Standard Serial Number (EISSN)
- 1525-2191
Digital Object Identifier (DOI)
- 10.2353/ajpath.2009.080223
Language
- eng
Conference Location
- United States