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Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells.

Publication ,  Journal Article
Iwata, Y; Matsushita, T; Horikawa, M; Dilillo, DJ; Yanaba, K; Venturi, GM; Szabolcs, PM; Bernstein, SH; Magro, CM; Williams, AD; Hall, RP ...
Published in: Blood
January 13, 2011

Regulatory B cells control inflammation and autoimmunity in mice, including the recently identified IL-10-competent B10 cell subset that represents 1% to 3% of spleen B cells. In this study, a comparable IL-10-competent B10 cell subset was characterized in human blood. B10 cells were functionally identified by their ability to express cytoplasmic IL-10 after 5 hours of ex vivo stimulation, whereas progenitor B10 (B10pro) cells required 48 hours of in vitro stimulation before they acquired the ability to express IL-10. B10 and B10pro cells represented 0.6% and approximately 5% of blood B cells, respectively. Ex vivo B10 and B10pro cells were predominantly found within the CD24(hi)CD27(+) B-cell subpopulation that was able to negatively regulate monocyte cytokine production through IL-10-dependent pathways during in vitro functional assays. Blood B10 cells were present in 91 patients with rheumatoid arthritis, systemic lupus erythematosus, primary Sjögren syndrome, autoimmune vesiculobullous skin disease, or multiple sclerosis, and were expanded in some cases as occurs in mice with autoimmune disease. Mean B10 + B10pro-cell frequencies were also significantly higher in patients with autoimmune disease compared with healthy controls. The characterization of human B10 cells will facilitate their identification and the study of their regulatory activities during human disease.

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Published In

Blood

DOI

EISSN

1528-0020

Publication Date

January 13, 2011

Volume

117

Issue

2

Start / End Page

530 / 541

Location

United States

Related Subject Headings

  • Young Adult
  • Precursor Cells, B-Lymphoid
  • Middle Aged
  • Mice
  • Interleukin-10
  • Immunology
  • Humans
  • Fluorescent Antibody Technique
  • Flow Cytometry
  • Cell Separation
 

Citation

APA
Chicago
ICMJE
MLA
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Iwata, Y., Matsushita, T., Horikawa, M., Dilillo, D. J., Yanaba, K., Venturi, G. M., … Tedder, T. F. (2011). Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells. Blood, 117(2), 530–541. https://doi.org/10.1182/blood-2010-07-294249
Iwata, Yohei, Takashi Matsushita, Mayuka Horikawa, David J. Dilillo, Koichi Yanaba, Guglielmo M. Venturi, Paul M. Szabolcs, et al. “Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells.Blood 117, no. 2 (January 13, 2011): 530–41. https://doi.org/10.1182/blood-2010-07-294249.
Iwata Y, Matsushita T, Horikawa M, Dilillo DJ, Yanaba K, Venturi GM, et al. Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells. Blood. 2011 Jan 13;117(2):530–41.
Iwata, Yohei, et al. “Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells.Blood, vol. 117, no. 2, Jan. 2011, pp. 530–41. Pubmed, doi:10.1182/blood-2010-07-294249.
Iwata Y, Matsushita T, Horikawa M, Dilillo DJ, Yanaba K, Venturi GM, Szabolcs PM, Bernstein SH, Magro CM, Williams AD, Hall RP, St Clair EW, Tedder TF. Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells. Blood. 2011 Jan 13;117(2):530–541.

Published In

Blood

DOI

EISSN

1528-0020

Publication Date

January 13, 2011

Volume

117

Issue

2

Start / End Page

530 / 541

Location

United States

Related Subject Headings

  • Young Adult
  • Precursor Cells, B-Lymphoid
  • Middle Aged
  • Mice
  • Interleukin-10
  • Immunology
  • Humans
  • Fluorescent Antibody Technique
  • Flow Cytometry
  • Cell Separation