L-selectin is dispensable for T regulatory cell function postallogeneic bone marrow transplantation.

Published

Journal Article

In murine models, the adoptive transfer of CD4(+) /CD25(+) regulatory T cells (T(regs) ) inhibited graft-versus-host disease (GvHD). Previous work has indicated a critical role for the adhesion molecule L-selectin (CD62L) in the function of T(regs) in preventing GvHD. Here we examined the capacity of naive wild-type (WT), CD62L(-/-) and ex vivo expanded CD62L(Lo) T(regs) to inhibit acute GvHD. Surprisingly, we found that CD62L(-/-) T(regs) were potent suppressors of GvHD, whereas CD62L(Lo) T(regs) were unable to inhibit disease despite being functionally competent to suppress allo T cell responses in vitro. Concomitant with improved outcomes, WT and CD62L(-/-) T(regs) significantly reduced liver pathology and systemic pro-inflammatory cytokine production, although CD62L(-/-) T(regs) were less effective in reducing lung pathology. While accumulation of CD62L(-/-) T(regs) in GvHD target organs was equivalent to WT T(regs) , CD62L(-/-) T(regs) did not migrate as well as WT T(regs) to peripheral lymph nodes (PLNs) over the first 2 weeks posttransplantation. This work demonstrated that CD62L was dispensable for T(reg) -mediated protection from GvHD.

Full Text

Duke Authors

Cited Authors

  • Carlson, MJ; Fulton, LM; Coghill, JM; West, ML; Burgents, JE; Wan, Y; Panoskaltsis-Mortari, A; Tedder, TF; Blazar, BR; Serody, JS

Published Date

  • December 2010

Published In

Volume / Issue

  • 10 / 12

Start / End Page

  • 2596 - 2603

PubMed ID

  • 21070606

Pubmed Central ID

  • 21070606

Electronic International Standard Serial Number (EISSN)

  • 1600-6143

Digital Object Identifier (DOI)

  • 10.1111/j.1600-6143.2010.03319.x

Language

  • eng

Conference Location

  • United States