Forces for morphogenesis investigated with laser microsurgery and quantitative modeling.

Journal Article

We investigated the forces that connect the genetic program of development to morphogenesis in Drosophila. We focused on dorsal closure, a powerful model system for development and wound healing. We found that the bulk of progress toward closure is driven by contractility in supracellular "purse strings" and in the amnioserosa, whereas adhesion-mediated zipping coordinates the forces produced by the purse strings and is essential only for the end stages. We applied quantitative modeling to show that these forces, generated in distinct cells, are coordinated in space and synchronized in time. Modeling of wild-type and mutant phenotypes is predictive; although closure in myospheroid mutants ultimately fails when the cell sheets rip themselves apart, our analysis indicates that beta(PS) integrin has an earlier, important role in zipping.

Full Text

Duke Authors

Cited Authors

  • Hutson, MS; Tokutake, Y; Chang, M-S; Bloor, JW; Venakides, S; Kiehart, DP; Edwards, GS

Published Date

  • April 4, 2003

Published In

Volume / Issue

  • 300 / 5616

Start / End Page

  • 145 - 149

PubMed ID

  • 12574496

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.1079552

Language

  • eng

Conference Location

  • United States