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Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization.

Publication ,  Journal Article
Crawford, JM; Su, Z; Varlamova, O; Bresnick, AR; Kiehart, DP
Published in: European Journal of Cell Biology
March 2001

Microinjection of constitutively active Cdc42 (V12Cdc42) disrupts the actomyosin cytoskeleton during cellularization (Crawford et al., Dev. Biol., 204, 151-164 (1998)). The p21-activated kinase (PAK) family of Ser/Thr kinases are effectors of GTP-bound forms of the small GTPases, Cdc42 and Rac. Drosophila PAK, which colocalizes with actin and myosin-II during cellularization, concentrates at sites of V12Cdc42-induced actomyosin disruption. In vitro biochemical analyses demonstrate that PAK phosphorylates the regulatory light chain (RLC) of Drosophila nonmuscle myosin-II on Ser21, a site known to activate myosin-II function. Although activated PAK does not disrupt the actomyosin cytoskeleton, it induces increased levels of Ser21 phosphorylated RLC. These findings suggest that increased levels of RLC phosphorylation do not contribute to disruption of the actomyosin hexagonal array.

Duke Scholars

Published In

European Journal of Cell Biology

Publication Date

March 2001

Volume

80

Issue

3

Start / End Page

240 / 244

Location

germany

Related Subject Headings

  • cdc42 GTP-Binding Protein
  • Time Factors
  • Serine
  • Protein Serine-Threonine Kinases
  • Protein Binding
  • Phosphorylation
  • Myosins
  • Microscopy, Fluorescence
  • Guanosine Triphosphate
  • Drosophila
 

Citation

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Crawford, J. M., Su, Z., Varlamova, O., Bresnick, A. R., & Kiehart, D. P. (2001). Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization. European Journal of Cell Biology, 80(3), 240–244.
Crawford, J. M., Z. Su, O. Varlamova, A. R. Bresnick, and D. P. Kiehart. “Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization.European Journal of Cell Biology 80, no. 3 (March 2001): 240–44.
Crawford JM, Su Z, Varlamova O, Bresnick AR, Kiehart DP. Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization. European Journal of Cell Biology. 2001 Mar;80(3):240–4.
Crawford, J. M., et al. “Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization.European Journal of Cell Biology, vol. 80, no. 3, Mar. 2001, pp. 240–44.
Crawford JM, Su Z, Varlamova O, Bresnick AR, Kiehart DP. Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization. European Journal of Cell Biology. 2001 Mar;80(3):240–244.

Published In

European Journal of Cell Biology

Publication Date

March 2001

Volume

80

Issue

3

Start / End Page

240 / 244

Location

germany

Related Subject Headings

  • cdc42 GTP-Binding Protein
  • Time Factors
  • Serine
  • Protein Serine-Threonine Kinases
  • Protein Binding
  • Phosphorylation
  • Myosins
  • Microscopy, Fluorescence
  • Guanosine Triphosphate
  • Drosophila