Skip to main content

Second-site noncomplementation identifies genomic regions required for Drosophila nonmuscle myosin function during morphogenesis.

Publication ,  Journal Article
Halsell, SR; Kiehart, DP
Published in: Genetics
April 1998

Drosophila is an ideal metazoan model system for analyzing the role of nonmuscle myosin-II (henceforth, myosin) during development. In Drosophila, myosin function is required for cytokinesis and morphogenesis driven by cell migration and/or cell shape changes during oogenesis, embryogenesis, larval development and pupal metamorphosis. The mechanisms that regulate myosin function and the supramolecular structures into which myosin incorporates have not been systematically characterized. The genetic screens described here identify genomic regions that uncover loci that facilitate myosin function. The nonmuscle myosin heavy chain is encoded by a single locus, zipper. Contiguous chromosomal deficiencies that represent approximately 70% of the euchromatic genome were screened for genetic interactions with two recessive lethal alleles of zipper in a second-site noncomplementation assay for the malformed phenotype. Malformation in the adult leg reflects aberrations in cell shape changes driven by myosin-based contraction during leg morphogenesis. Of the 158 deficiencies tested, 47 behaved as second-site noncomplementors of zipper. Two of the deficiencies are strong interactors, 17 are intermediate and 28 are weak. Finer genetic mapping reveals that mutations in cytoplasmic tropomyosin and viking (collagen IV) behave as second-site noncomplementors of zipper during leg morphogenesis and that zipper function requires a previously uncharacterized locus, E3.10/J3.8, for leg morphogenesis and viability.

Duke Scholars

Published In

Genetics

DOI

EISSN

1943-2631

ISSN

0016-6731

Publication Date

April 1998

Volume

148

Issue

4

Start / End Page

1845 / 1863

Related Subject Headings

  • X Chromosome
  • Myosin Heavy Chains
  • Morphogenesis
  • Membrane Proteins
  • Genetic Complementation Test
  • Drosophila Proteins
  • Drosophila
  • Developmental Biology
  • Chromosome Aberrations
  • Binding Sites
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Halsell, S. R., & Kiehart, D. P. (1998). Second-site noncomplementation identifies genomic regions required for Drosophila nonmuscle myosin function during morphogenesis. Genetics, 148(4), 1845–1863. https://doi.org/10.1093/genetics/148.4.1845
Halsell, S. R., and D. P. Kiehart. “Second-site noncomplementation identifies genomic regions required for Drosophila nonmuscle myosin function during morphogenesis.Genetics 148, no. 4 (April 1998): 1845–63. https://doi.org/10.1093/genetics/148.4.1845.
Halsell, S. R., and D. P. Kiehart. “Second-site noncomplementation identifies genomic regions required for Drosophila nonmuscle myosin function during morphogenesis.Genetics, vol. 148, no. 4, Apr. 1998, pp. 1845–63. Epmc, doi:10.1093/genetics/148.4.1845.

Published In

Genetics

DOI

EISSN

1943-2631

ISSN

0016-6731

Publication Date

April 1998

Volume

148

Issue

4

Start / End Page

1845 / 1863

Related Subject Headings

  • X Chromosome
  • Myosin Heavy Chains
  • Morphogenesis
  • Membrane Proteins
  • Genetic Complementation Test
  • Drosophila Proteins
  • Drosophila
  • Developmental Biology
  • Chromosome Aberrations
  • Binding Sites