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Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function.

Publication ,  Journal Article
Young, PE; Richman, AM; Ketchum, AS; Kiehart, DP
Published in: Genes & development
January 1993

We provide the first link between a known molecular motor and morphogenesis, the fundamental process of cell shape changes and movements that characterizes development throughout phylogeny. By reverse genetics, we generate mutations in the Drosophila conventional nonmuscle myosin (myosin II) heavy chain gene and show that this gene is essential. We demonstrate that these mutations are allelic to previously identified, recessive, embryonic-lethal zipper mutations and thereby identify nonmuscle myosin heavy chain as the zipper gene product. Embryos that lack functional myosin display defects in dorsal closure, head involution, and axon patterning. Analysis of cell morphology and myosin localization during dorsal closure in wild-type and homozygous mutant embryos demonstrates a key role for myosin in the maintenance of cell shape and suggests a model for the involvement of myosin in cell sheet movement during development. Our experiments, in conjunction with the observation that cytokinesis also requires myosin, suggest that the processes of cell shape change in morphogenesis and cell division are intimately and mechanistically related.

Duke Scholars

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Published In

Genes & development

DOI

EISSN

1549-5477

ISSN

0890-9369

Publication Date

January 1993

Volume

7

Issue

1

Start / End Page

29 / 41

Related Subject Headings

  • Myosins
  • Mutation
  • Muscles
  • Morphogenesis
  • Molecular Sequence Data
  • Male
  • Immunoblotting
  • Genetic Complementation Test
  • Female
  • Drosophila
 

Citation

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Young, P. E., Richman, A. M., Ketchum, A. S., & Kiehart, D. P. (1993). Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function. Genes & Development, 7(1), 29–41. https://doi.org/10.1101/gad.7.1.29
Young, P. E., A. M. Richman, A. S. Ketchum, and D. P. Kiehart. “Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function.Genes & Development 7, no. 1 (January 1993): 29–41. https://doi.org/10.1101/gad.7.1.29.
Young PE, Richman AM, Ketchum AS, Kiehart DP. Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function. Genes & development. 1993 Jan;7(1):29–41.
Young, P. E., et al. “Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function.Genes & Development, vol. 7, no. 1, Jan. 1993, pp. 29–41. Epmc, doi:10.1101/gad.7.1.29.
Young PE, Richman AM, Ketchum AS, Kiehart DP. Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function. Genes & development. 1993 Jan;7(1):29–41.

Published In

Genes & development

DOI

EISSN

1549-5477

ISSN

0890-9369

Publication Date

January 1993

Volume

7

Issue

1

Start / End Page

29 / 41

Related Subject Headings

  • Myosins
  • Mutation
  • Muscles
  • Morphogenesis
  • Molecular Sequence Data
  • Male
  • Immunoblotting
  • Genetic Complementation Test
  • Female
  • Drosophila