Characterization of the light-responsive promoter of rice chloroplast psbD-C operon and the sequence-specific DNA binding factor.

Published

Journal Article

The transcription of rice plastid psbD-psbC genes encoding photosystem II reaction center protein D2 and chlorophyll alpha-binding protein CP43 is closely regulated by light. To elucidate the sequence requirement for the light-responsive promoter of psbD-psbC operon, transcriptional analysis of the rice promoter was performed with deleted mutants and site-directed mutants in vitro. Deletion of -546 approximately -100 upstream sequences resulted in 4- to 5-fold decrease in the transcription rate. Further deletion of -99 approximately -40 conserved region of repeated sequences resulted in 2-fold decrease in the transcription rate. The core light-responsive promoter requires "-10" element but not "-35" element for accurate initiation of basal transcription. No downstream promoter element was found in the +4 approximately +111 region. The competitive gel-retardation experiments revealed the presence of DNA-binding protein in the rice chloroplasts, which interacts specifically with the -60 approximately -37 repeated sequences. Southwestern blot analysis further demonstrated that the binding factor is composed of 36-kDa polypeptide(s).

Full Text

Cited Authors

  • To, KY; Cheng, MC; Suen, DF; Mon, DP; Chen, LF; Chen, SC

Published Date

  • July 1996

Published In

Volume / Issue

  • 37 / 5

Start / End Page

  • 660 - 666

PubMed ID

  • 8819312

Pubmed Central ID

  • 8819312

Electronic International Standard Serial Number (EISSN)

  • 1471-9053

International Standard Serial Number (ISSN)

  • 0032-0781

Digital Object Identifier (DOI)

  • 10.1093/oxfordjournals.pcp.a028995

Language

  • eng