Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells.

Published

Journal Article

The objective of this study was to investigate the antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A (ConA) on human melanoma A375 cells. We firstly simulated the three-dimensional structure of ConA. Subsequently, we found that ConA possessed remarkable antiproliferative effect on A375 cells. Further experimental data indicated that there was a link between its hemagglutinating activity, mannose-binding activity and antiproliferative activity. In addition, we showed that ConA induced A375 cell apoptosis in a caspase-dependent manner. Then, we demonstrated that the treatment of ConA caused mitochondrial transmembrane potential (MMP) collapse, cytochrome c release and caspase activation. In conclusion, we report for the first time that there may be a close correlation between carbohydrate-binding activity of ConA and its antiproliferative activity. Also, we demonstrate firstly that ConA induces A375 cell death in a caspase-dependent manner as well as through a mitochondrial apoptotic pathway.

Full Text

Cited Authors

  • Liu, B; Li, C-Y; Bian, H-J; Min, M-W; Chen, L-F; Bao, J-K

Published Date

  • February 2009

Published In

Volume / Issue

  • 482 / 1-2

Start / End Page

  • 1 - 6

PubMed ID

  • 19111670

Pubmed Central ID

  • 19111670

Electronic International Standard Serial Number (EISSN)

  • 1096-0384

International Standard Serial Number (ISSN)

  • 0003-9861

Digital Object Identifier (DOI)

  • 10.1016/j.abb.2008.12.003

Language

  • eng