Effect of sex on the induction of anti-DNA antibodies in normal mice immunized with bacterial DNA.

Journal Article (Journal Article)

Immunization of normal mice with bacterial DNA elicits a significant IgG anti-DNA response and has been explored as a model of systemic lupus erythematosus. To determine whether this induced response is influenced by sex, we have measured anti-DNA levels in normal male and female BALB/c mice immunized with single stranded DNA from E. coli as complexes with methylated bovine serum albumin (mBSA) in adjuvant. By ELISA assays, anti-DNA levels of immunized females were approximately 16-fold higher than those of immunized males; levels of antibodies to the mBSA carrier were similar, however. The antibodies from females and males showed a similar degree of cross-reactivity when assayed using other natural and synthetic DNA antigens, including mammalian DNA. These findings suggest the potentiation of anti-DNA production in females by antigen-specific mechanisms and provide further evidence that immunization with bacterial DNA replicates features of autoantibody production in SLE.

Full Text

Duke Authors

Cited Authors

  • Palmer, SM; Gilkeson, GS; Pisetsky, DS

Published Date

  • August 1993

Published In

Volume / Issue

  • 2 / 4

Start / End Page

  • 251 - 255

PubMed ID

  • 8268973

International Standard Serial Number (ISSN)

  • 0961-2033

Digital Object Identifier (DOI)

  • 10.1177/096120339300200408


  • eng

Conference Location

  • England