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Analysis of the expression of CD5 by human B cells and correlation with functional activity.

Publication ,  Journal Article
Vernino, LA; Pisetsky, DS; Lipsky, PE
Published in: Cell Immunol
January 1992

B cells expressing the CD5 marker in the mouse have been suggested to be a separate lineage and a major source of autoantibody production. In man, this relationship is less clear. Studies were therefore undertaken to determine whether human CD5+ B cells represent a distinct lineage of cells that differ in patterns of antibody production from CD5- B cells. In normal B cell populations, CD5 was expressed by a mean of 24.0 +/- 2.8% (n = 10) of CD20+ B cells. Of note, an increased frequency of CD5+ B cells was not found in patients with systemic lupus erythematosus (mean of 17.9 +/- 2.8%, n = 16). Analyzing CD5+ B cells for cell membrane Ig isotype expression demonstrated similar frequencies of IgG and IgA expressing cells as were found on the CD5- B cell population, although the frequency of IgM+ cells was slightly increased. Incubation of CD20+ B cells with phorbol myristate acetate (PMA) for 72 hr increased the frequency of CD5 expressing B cells by more than threefold. CD5 expression was also increased by coculture with anti-CD3-activated T cells and most markedly by simultaneous stimulation with both PMA- and anti-CD3-activated T cells (greater than 50% positive). Analysis of CD5- B cells clearly indicated that stimulation with PMA or anti-CD3-activated T cells induced the majority to become CD5+ transiently. Functional analysis of Ig production by CD5+ and CD5- B cells stimulated with anti-CD3-activated T cells indicated that both populations in normals produced IgM and a variety of autoantibodies in comparable amounts, whereas the CD5- B cells produced greater quantities of IgG. B cells were activated with anti-CD3-stimulated T cells followed by separation into CD5+ and CD5- populations. The largest amount of Ig was produced by CD5- B cells that were induced to express CD5, although all populations produced some Ig. These data suggest that CD5 behaves as an activation marker on human B cells rather than as a marker for a distinct lineage of cells. Moreover, CD5 expression does not appear to identify a population of resting B cells with a greater capacity to produce antibodies to DNA or other autoantibodies.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

January 1992

Volume

139

Issue

1

Start / End Page

185 / 197

Location

Netherlands

Related Subject Headings

  • Lymphocyte Activation
  • Leukocyte Count
  • In Vitro Techniques
  • Immunology
  • Humans
  • Flow Cytometry
  • Cell Differentiation
  • CD5 Antigens
  • B-Lymphocyte Subsets
  • Autoantibodies
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Vernino, L. A., Pisetsky, D. S., & Lipsky, P. E. (1992). Analysis of the expression of CD5 by human B cells and correlation with functional activity. Cell Immunol, 139(1), 185–197. https://doi.org/10.1016/0008-8749(92)90111-2
Vernino, L. A., D. S. Pisetsky, and P. E. Lipsky. “Analysis of the expression of CD5 by human B cells and correlation with functional activity.Cell Immunol 139, no. 1 (January 1992): 185–97. https://doi.org/10.1016/0008-8749(92)90111-2.
Vernino LA, Pisetsky DS, Lipsky PE. Analysis of the expression of CD5 by human B cells and correlation with functional activity. Cell Immunol. 1992 Jan;139(1):185–97.
Vernino, L. A., et al. “Analysis of the expression of CD5 by human B cells and correlation with functional activity.Cell Immunol, vol. 139, no. 1, Jan. 1992, pp. 185–97. Pubmed, doi:10.1016/0008-8749(92)90111-2.
Vernino LA, Pisetsky DS, Lipsky PE. Analysis of the expression of CD5 by human B cells and correlation with functional activity. Cell Immunol. 1992 Jan;139(1):185–197.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

January 1992

Volume

139

Issue

1

Start / End Page

185 / 197

Location

Netherlands

Related Subject Headings

  • Lymphocyte Activation
  • Leukocyte Count
  • In Vitro Techniques
  • Immunology
  • Humans
  • Flow Cytometry
  • Cell Differentiation
  • CD5 Antigens
  • B-Lymphocyte Subsets
  • Autoantibodies