Idiotypic analysis of a monoclonal anti-Sm antibody.

Published

Journal Article

Among murine models of autoimmunity, MRL mice are unique in their expression of antibodies to the nuclear antigen Sm. To assess genetic mechanisms in the control of this response, the idiotypes borne by a monoclonal anti-Sm antibody of MRL-Ipr/Ipr origin were investigated. Rabbit antisera were prepared against Y2, a hybridoma product with anti-Sm activity, and were rendered specific for idiotype by extensive absorption with normal globulins from BALB/c mice. In assays of idiotype by an inhibition ELISA, Y2 was shown to share idiotypes with Y12, another monoclonal anti-Sm derived from the same fusion as Y2; other monoclonal autoantibodies of MRL origin but different antigenic specificity failed to display idiotype activity in this assay. The presence of other anti-idiotypic specificities was revealed by absorption and elution of the anti-idiotype from an MRL globulin column; sera from both anti-Sm-positive and negative mice demonstrated these idiotypes. These results suggest that the predominant specificities detected by the anti-idiotype were unique to the monoclonal antibodies of the same animal, although there was also activity to idiotypes not related to anti-Sm binding molecules.

Full Text

Duke Authors

Cited Authors

  • Pisetsky, DS; Lerner, EA

Published Date

  • October 1, 1982

Published In

Volume / Issue

  • 129 / 4

Start / End Page

  • 1489 - 1492

PubMed ID

  • 6180012

Pubmed Central ID

  • 6180012

International Standard Serial Number (ISSN)

  • 0022-1767

Language

  • eng

Conference Location

  • United States