Stimulation of murine lymphocyte proliferation by a phosphorothioate oligonucleotide with antisense activity for herpes simplex virus.

Published

Journal Article

To investigate further the immunological properties of nucleic acids, the mitogenicity of a phosphorothioate oligonucleotide (S-oligo 1082) with antisense activity for herpes simplex virus was tested. This compound stimulated proliferation and antibody production by murine lymphocytes in in vitro cultures. Proliferation was dose-dependent and unaffected by T cell depletion. Furthermore, inclusion of a non-mitogenic DNA in the medium did not block stimulation. Since 1082 does not have homology to a known gene involved in lymphocyte activation, these observations suggest that S-oligo antisense compounds may display non-specific activating effects, at least on murine B cells.

Full Text

Duke Authors

Cited Authors

  • Pisetsky, DS; Reich, CF

Published Date

  • 1994

Published In

Volume / Issue

  • 54 / 2

Start / End Page

  • 101 - 107

PubMed ID

  • 8277816

Pubmed Central ID

  • 8277816

International Standard Serial Number (ISSN)

  • 0024-3205

Digital Object Identifier (DOI)

  • 10.1016/0024-3205(94)00780-2

Language

  • eng

Conference Location

  • Netherlands