Specificity of anti-DNA antibodies induced in normal mice by immunization with bacterial DNA.

Published

Journal Article

To determine the specificity of anti-DNA antibodies induced in normal mice by immunization with bacterial DNA, sera from BALB/c mice immunized with single-stranded DNA from Escherichia coli (EC) were tested for binding to a panel of synthetic DNA and RNA homopolymers as well as duplexes. Results of these studies indicate that sera from EC DNA immunized mice preferentially bind certain DNA and RNA homopolymers as well as DNA duplexes. Furthermore, the specificity of the antibodies from immunized mice resembled those of sera from autoimmune MRL-lpr/lpr mice in terms of the synthetic antigens recognized, although some differences were noted in the magnitude of the response to individual duplexes. These results suggest that anti-DNA antibodies induced by bacterial DNA bind to DNA structures dependent on both the base and the sugar phosphate moieties of the nucleic acid antigen and may resemble some anti-DNA antibodies expressed in spontaneous autoimmune disease in these binding properties.

Full Text

Duke Authors

Cited Authors

  • Gilkeson, GS; Pritchard, AJ; Pisetsky, DS

Published Date

  • May 1991

Published In

Volume / Issue

  • 59 / 2

Start / End Page

  • 288 - 300

PubMed ID

  • 1706971

Pubmed Central ID

  • 1706971

International Standard Serial Number (ISSN)

  • 0090-1229

Language

  • eng

Conference Location

  • United States