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The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis.

Publication ,  Journal Article
Guiducci, S; Distler, JHW; Jüngel, A; Huscher, D; Huber, LC; Michel, BA; Gay, RE; Pisetsky, DS; Gay, S; Matucci-Cerinic, M; Distler, O
Published in: Arthritis Rheum
September 2008

OBJECTIVE: Microparticles are small, membrane-coated vesicles that can serve as novel signaling structures between cells. The aim of the present study was to analyze the profile of microparticles in the blood of patients with systemic sclerosis (SSc; scleroderma) and healthy controls. METHODS: The study population consisted of 37 patients with SSc and 15 healthy subjects of comparable sex and age. Microparticles were isolated from plasma by high-speed differential centrifugation. Microparticles were stained with monoclonal antibodies against cell type-specific markers and were quantified by fluorescence-activated cell sorting analyses. RESULTS: The total number of microparticles was strongly increased in patients with SSc compared with healthy controls (mean +/- SEM 88.0 +/- 4.8 x 10(5) microparticles/ml plasma versus 42.3 +/- 9.4 x 10(5) microparticles/ml plasma; P < 0.001). Similarly, significant increases were found for microparticles derived from platelets, endothelial cells, monocytes, and T cells, reflecting the activation of these cells in SSc. Platelets were the most common source of microparticles in the blood of patients with SSc (66.9 +/- 5.2% of all microparticles) and healthy donors, followed by microparticles derived from endothelial cells (8.8 +/- 0.9% in SSc patients). The modified Rodnan skin thickness score (MRSS) was inversely correlated with the total number of microparticles. Furthermore, patients with cutaneous ulcers showed a significantly lower total number of microparticles. In multivariate analysis, an additive model of age, C-reactive protein, MRSS, and subtype of disease accounted for 55% of the variability of the total microparticle count (r = 0.744). CONCLUSION: The number of microparticles from different cellular sources is increased in the blood of SSc patients. Considering their role as important mediators of intercellular communication, microparticles could be a novel link between activated cellular compartments in the pathogenesis of SSc.

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Published In

Arthritis Rheum

DOI

EISSN

1529-0131

Publication Date

September 2008

Volume

58

Issue

9

Start / End Page

2845 / 2853

Location

United States

Related Subject Headings

  • Statistics, Nonparametric
  • Severity of Illness Index
  • Scleroderma, Systemic
  • Regression Analysis
  • Plasma
  • Multivariate Analysis
  • Middle Aged
  • Male
  • Humans
  • Flow Cytometry
 

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Guiducci, S., Distler, J. H. W., Jüngel, A., Huscher, D., Huber, L. C., Michel, B. A., … Distler, O. (2008). The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis. Arthritis Rheum, 58(9), 2845–2853. https://doi.org/10.1002/art.23735
Guiducci, Serena, Jörg H. W. Distler, Astrid Jüngel, Dörte Huscher, Lars C. Huber, Beat A. Michel, Renate E. Gay, et al. “The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis.Arthritis Rheum 58, no. 9 (September 2008): 2845–53. https://doi.org/10.1002/art.23735.
Guiducci S, Distler JHW, Jüngel A, Huscher D, Huber LC, Michel BA, et al. The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis. Arthritis Rheum. 2008 Sep;58(9):2845–53.
Guiducci, Serena, et al. “The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis.Arthritis Rheum, vol. 58, no. 9, Sept. 2008, pp. 2845–53. Pubmed, doi:10.1002/art.23735.
Guiducci S, Distler JHW, Jüngel A, Huscher D, Huber LC, Michel BA, Gay RE, Pisetsky DS, Gay S, Matucci-Cerinic M, Distler O. The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis. Arthritis Rheum. 2008 Sep;58(9):2845–2853.
Journal cover image

Published In

Arthritis Rheum

DOI

EISSN

1529-0131

Publication Date

September 2008

Volume

58

Issue

9

Start / End Page

2845 / 2853

Location

United States

Related Subject Headings

  • Statistics, Nonparametric
  • Severity of Illness Index
  • Scleroderma, Systemic
  • Regression Analysis
  • Plasma
  • Multivariate Analysis
  • Middle Aged
  • Male
  • Humans
  • Flow Cytometry