Induction of immune activation by a novel immunomodulatory oligonucleotide without thymocyte apoptosis.

Published

Journal Article

Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs (CpG DNA) can potently stimulate innate immunity. While the actions of CpG DNA resemble those of LPS, these molecules stimulate distinct Toll-like receptors as well as cell types. In a previous study, we showed that a CpG ODN could induce cytokine production but, unlike LPS, did not induce thymocyte apoptosis. In this study, we have further investigated these differences using as a model a second-generation immunostimulatory oligonucleotide called HYB2048. Following administration to normal BALB/c mice, HYB2048-induced IL-12 but not IL-6 production. Under conditions in which LPS induced thymocyte apoptosis, HYB2048 did not cause significant cell death and, furthermore, did not block apoptosis induced by LPS. The levels of corticosterone induced by HYB2048 were also significantly lower than those induced by LPS. This pattern of activation could distinguish CpG DNA from LPS in its effects on the immune system.

Full Text

Duke Authors

Cited Authors

  • Jiang, W; Reich, CF; You, D; Kandimalla, E; Agrawal, S; Pisetsky, DS

Published Date

  • May 21, 2004

Published In

Volume / Issue

  • 318 / 1

Start / End Page

  • 60 - 66

PubMed ID

  • 15110753

Pubmed Central ID

  • 15110753

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/j.bbrc.2004.04.001

Language

  • eng

Conference Location

  • United States