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The release of microparticles by Jurkat leukemia T cells treated with staurosporine and related kinase inhibitors to induce apoptosis.

Publication ,  Journal Article
Ullal, AJ; Pisetsky, DS
Published in: Apoptosis
May 2010

Microparticles (MPs) are small membrane-bound vesicles released from cells undergoing activation or cell death. These particles display potent biological activities that can impact on physiologic and pathologic processes. Previous studies with the Jurkat T leukemia cell line demonstrated that staurosporine (STS) induces the release of MPs as cells undergo apoptosis. To investigate further this process, we tested the effects of STS, its analogue, 7-hydroxystaurosporine (UCN-01), and other protein kinase C (PKC) and cyclin-dependent kinase (CDK) inhibitors. FACS analysis was used to assess MP release. Results of these studies indicate that STS and UCN-01 induce MP release by Jurkat cells; in contrast, other PKC and CDK inhibitors failed to induce comparable release, suggesting that release does not result from simple inhibition of either kinase alone. Time course experiments indicated that STS-induced particle release occurred as early as 2 h after treatment, with the early release MPs displaying low levels of binding of annexin V and propidium iodide (PI). Early-release MPs, however, matured in culture to an annexin V- and PI-positive phenotype. Together, these results indicate that STS and UCN-01 induce MPs that are phenotypically distinct and reflect specific patterns of kinase inhibition during apoptosis.

Duke Scholars

Published In

Apoptosis

DOI

EISSN

1573-675X

Publication Date

May 2010

Volume

15

Issue

5

Start / End Page

586 / 596

Location

Netherlands

Related Subject Headings

  • Staurosporine
  • Protein Kinase C
  • Nucleic Acids
  • Jurkat Cells
  • Humans
  • Flow Cytometry
  • Enzyme Inhibitors
  • Cell-Derived Microparticles
  • Biochemistry & Molecular Biology
  • Apoptosis
 

Citation

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ICMJE
MLA
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Ullal, A. J., & Pisetsky, D. S. (2010). The release of microparticles by Jurkat leukemia T cells treated with staurosporine and related kinase inhibitors to induce apoptosis. Apoptosis, 15(5), 586–596. https://doi.org/10.1007/s10495-010-0470-3
Ullal, Anirudh J., and David S. Pisetsky. “The release of microparticles by Jurkat leukemia T cells treated with staurosporine and related kinase inhibitors to induce apoptosis.Apoptosis 15, no. 5 (May 2010): 586–96. https://doi.org/10.1007/s10495-010-0470-3.
Ullal, Anirudh J., and David S. Pisetsky. “The release of microparticles by Jurkat leukemia T cells treated with staurosporine and related kinase inhibitors to induce apoptosis.Apoptosis, vol. 15, no. 5, May 2010, pp. 586–96. Pubmed, doi:10.1007/s10495-010-0470-3.
Journal cover image

Published In

Apoptosis

DOI

EISSN

1573-675X

Publication Date

May 2010

Volume

15

Issue

5

Start / End Page

586 / 596

Location

Netherlands

Related Subject Headings

  • Staurosporine
  • Protein Kinase C
  • Nucleic Acids
  • Jurkat Cells
  • Humans
  • Flow Cytometry
  • Enzyme Inhibitors
  • Cell-Derived Microparticles
  • Biochemistry & Molecular Biology
  • Apoptosis