Structure and function of anti-DNA autoantibodies derived from a single autoimmune mouse.

Journal Article (Journal Article)

Four monoclonal anti-DNA antibodies derived from a single autoimmune MRL/lpr mouse were studied. Three of these antibodies showed similarities in DNA binding; the fourth had a much higher specific activity for single-stranded DNA and, in addition, was unique in binding double-stranded DNA and cardiolipin. Complete nucleotide sequences of heavy- and light-chain variable regions demonstrated that all four antibodies are clonally related. The sequences also showed numerous somatic mutations, the distribution of which suggests that positive selection by antigen operated on these clonally related autoantibodies.

Full Text

Duke Authors

Cited Authors

  • Shlomchik, MJ; Aucoin, AH; Pisetsky, DS; Weigert, MG

Published Date

  • December 1987

Published In

Volume / Issue

  • 84 / 24

Start / End Page

  • 9150 - 9154

PubMed ID

  • 3480535

Pubmed Central ID

  • PMC299710

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.84.24.9150


  • eng

Conference Location

  • United States