IgG binding of monoclonal anti-nuclear antibodies from MRL-lpr/lpr mice.

Published

Journal Article

To assess the specificity of anti-nuclear antibodies with cross-reactive rheumatoid factor (RF) activity, monoclonal anti-DNA and anti-Sm antibodies from MRL-lpr/lpr mice were tested for binding to a variety of IgG antigens. These antibodies had all been previously identified as binding heterologous IgG. By ELISA, antibodies in this panel all bound BALB/c myeloma proteins representing the different IgG subclasses, indicating broad reactivity with murine IgG as well as heterologous IgG. The determinant recognized by these antibodies was further investigated using the Fab, F(ab')2 and Fc fragments of both human as well as rabbit origin. All antibodies bound well to fragments as well as intact IgG antigens. These antibodies were further analysed by Western blotting, demonstrating that most bound to both heavy and light chains of human origin. Together, these observations suggest that some anti-nuclear antibodies bind a conserved antigenic determinant present widely on immunoglobulin chains. This determinant may represent a common sequence important in immunoglobulin domain structure.

Full Text

Duke Authors

Cited Authors

  • Pisetsky, DS; Darwin, BS; Reich, CF

Published Date

  • December 1990

Published In

Volume / Issue

  • 71 / 4

Start / End Page

  • 586 - 591

PubMed ID

  • 2279742

Pubmed Central ID

  • 2279742

International Standard Serial Number (ISSN)

  • 0019-2805

Language

  • eng

Conference Location

  • England